نتایج جستجو برای: pol

تعداد نتایج: 9633  

2013
Qiuying Yang Liande Li Zhihong Xue Qiaohong Ye Lin Zhang Shaojie Li Yi Liu

Most plant and animal microRNAs (miRNAs) are transcribed by RNA polymerase II. We previously discovered miRNA-like small RNAs (milRNAs) in the filamentous fungus Neurospora crassa and uncovered at least four different pathways for milRNA production. To understand the evolutionary origin of milRNAs, we determined the roles of polymerases II and III (Pol II and Pol III) in milRNA transcription. O...

2011
Jennifer M. Allen David M. Simcha Nolan G. Ericson David L. Alexander Jacob T. Marquette Benjamin P. Van Biber Chris J. Troll Rachel Karchin Jason H. Bielas Lawrence A. Loeb Manel Camps

DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions. However, it is unclear how pol I and pol III work together during replication and repair or how extensive pol I processing of Okazaki fragments is in vivo. Here, we address these questions by analyzing pol I mutations generated through error-prone replication of ColE1...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2015
Andrea F Moon Rajendrakumar A Gosavi Thomas A Kunkel Lars C Pedersen Katarzyna Bebenek

Among the many proteins used to repair DNA double-strand breaks by nonhomologous end joining (NHEJ) are two related family X DNA polymerases, Pol λ and Pol µ. Which of these two polymerases is preferentially used for filling DNA gaps during NHEJ partly depends on sequence complementarity at the break, with Pol λ and Pol µ repairing complementary and noncomplementary ends, respectively. To bette...

Journal: :Cell 2007
Claus-D. Kuhn Sebastian R. Geiger Sonja Baumli Marco Gartmann Jochen Gerber Stefan Jennebach Thorsten Mielke Herbert Tschochner Roland Beckmann Patrick Cramer

Synthesis of ribosomal RNA (rRNA) by RNA polymerase (Pol) I is the first step in ribosome biogenesis and a regulatory switch in eukaryotic cell growth. Here we report the 12 A cryo-electron microscopic structure for the complete 14-subunit yeast Pol I, a homology model for the core enzyme, and the crystal structure of the subcomplex A14/43. In the resulting hybrid structure of Pol I, A14/43, th...

2017
Louise E Kerry Elaine E Pegg Donald P Cameron James Budzak Gretchen Poortinga Katherine M Hannan Ross D Hannan Gloria Rudenko

Trypanosoma brucei relies on an essential Variant Surface Glycoprotein (VSG) coat for survival in the mammalian bloodstream. High VSG expression within an expression site body (ESB) is mediated by RNA polymerase I (Pol I), which in other eukaryotes exclusively transcribes ribosomal RNA genes (rDNA). As T. brucei is reliant on Pol I for VSG transcription, we investigated Pol I transcription inhi...

Journal: :Journal of virology 2012
Yuna Sun Yaxin Wang Chao Shan Cheng Chen Peng Xu Mohan Song Honggang Zhou Cheng Yang Wenbo Xu Pei-Yong Shi Bo Zhang Zhiyong Lou

VPg uridylylation is essential for picornavirus RNA replication. The VPg uridylylation reaction consists of the binding of VPg to 3D polymerase (3D(pol)) and the transfer of UMP by 3D(pol) to the hydroxyl group of the third amino acid Tyr of VPg. Previous studies suggested that different picornaviruses employ distinct mechanisms during VPg binding and uridylylation. Here, we report a novel site...

Journal: :Trends in biochemical sciences 2011
Magdalena Boguta Damian Graczyk

The synthesis of tRNA by yeast RNA polymerase III (Pol III) is regulated in response to changing environmental conditions. This control is mediated by Maf1, the global negative regulator of Pol III transcription conserved from yeast to humans. Details regarding the molecular basis of Pol III repression by Maf1 are now emerging from recently reported structural and biochemical data on Pol III an...

Journal: :Molecular cell 2012
Miki Jishage Sohail Malik Ulrich Wagner Beatrix Uberheide Yasushi Ishihama Xiaopeng Hu Brian T Chait Averell Gnatt Bing Ren Robert G Roeder

Pol II(G) is a distinct form of RNA polymerase II that contains the tightly associated Gdown1 polypeptide (encoded by POLR2M). Unlike Pol II, Pol II(G) is highly dependent upon Mediator for robust activator-dependent transcription in a biochemically defined in vitro system. Here, in vitro studies show that Gdown1 competes with TFIIF for binding to the RPB1 and RPB5 subunits of Pol II, thereby i...

Journal: :Molecular cell 2006
Anna J Jasiak Karim-Jean Armache Birgit Martens Ralf-Peter Jansen Patrick Cramer

We obtained an 11 subunit model of RNA polymerase (Pol) III by combining a homology model of the nine subunit core enzyme with a new X-ray structure of the subcomplex C17/25. Compared to Pol II, Pol III shows a conserved active center for RNA synthesis but a structurally different upstream face for specific initiation complex assembly during promoter selection. The Pol III upstream face include...

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