Search Pointers organize the main part of the application on the Internet. However, because of Information management hardware, high volume of data and word similarities in different fields the most answers to the user s’ questions aren`t correct. So the web graph clustering and cluster placement in corresponding answers helps user to achieve his or her intended results. Community (web communit...

Wang Bi’s work is often used as evidence for “Confucian” interpretations and translations of the Laozi. Those who argue that explicit rejections Confucian values in chapters 5, 18, 19, 38 should actually be read admonishing hollow imitation mere appearance morality cite Bi. Additionally, this great philosopher normally taken a commentator simply sought to synthesize Daoist ideas. In paper, I wi...

According to several passages in the available sources, Kant developed some ideas about difference between being persuaded and convinced which have antecedents German tradition, but with regard he of his own. It is my intention examine precisely these passages. I will explain how philosopher understood this difference, what its relevance why figure other necessary determine it. For purpose, tex...

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Purpose. This article provides a morphological study of the life Hryhorii Skovoroda as philosopher’s self-construction – compared to lives such philosophers Immanuel Kant, Friedrich Nietzsche, Martin Heidegger and Henry Thoreau. Theoretical basis. The is based on use monadological approach history in combination with biographical method. ideas classical philosophical systems Gottfried Leibniz O...