Quinolone antibiotics have been commonly used to treat cases of multiple antibiotic resistance. Unfortunately, quinolone so much resisted by infectious bacterial agents. This study aimed evaluate the susceptibility some clinical isolates E. coli and determination plasmid-encoded resistance genes.
 Our results showed plasmid quinolone-resistance genes in following prevalence: qnr genes: S (...

Listeria monocytogenes is a gram-positive, facultative intracellular bacterium implicated in severe food-borne illness (listeriosis) in humans. The construction of well-defined gene replacements in the genome of L. monocytogenes has been instrumental to several genetic studies of the virulence and other attributes of the organism. Construction of such mutations by currently available procedures...

tetanus is a disease caused by tetanus toxin, a potent inhibitor for the release of inhibitory neurotransmitter in the central nervous system that causes spastic paralysis. fragment c (52 kd) of this toxin is responsible for binding to the neuronal membrane. for this reason, and also its non toxigenic and immunogenic nature, this fragment might be ideal for new vaccine development. presently, w...

conclusions our findings revealed that the zfn technology could be employed to overcome ampicillin resistance by the targeted disruption of the ampicillin resistance gene, which leads to inactivation of β-lactam synthesis. therefore, zfn technology could be engaged to decrease the antibiotic resistance issue with the construction of a zfn archive against different args. to tackle the resistance...

pseudomonas aeruginosa is an important life-threatening nosocomial pathogen and plays a prominent role in serious infections in burned patients. the current study was undertaken to characterize p. aeruginosa strains isolated from burned patients in tehran, iran. the study was conducted in a major burn center in tehran, iran in 2007. a total of seventy specimens obtained from different clinical ...

background and purpose: brain-derived neurotrophic factor (bdnf) is present in the hippocampus and cerebral cortex in high concentrations and has an important role in development, maintenance and regeneration of the nervous system. the aim of this study was to produce active bdnf in e.coli using transforming plasmid. methods: to construct an expression plasmid of bdnf, a gene coding for it was ...

shigellosis is an acute gastroenteritis caused by shigella species. the purpose of this study was to determine plasmid profile, antibiotic resistance and phenotypic virulent by congo red between s. flexneri strains. the isolated bacteria were identified by standard bacterial and biochemical methods. plasmids were isolated by alkaline lysis method. antibiotic susceptibility test was performed ac...

A recombinant DNA plasmid library was constructed from HeLa cell extrachromosomal circular DNA and the sequence organization of one family of clones, which contain sequences enriched in HeLa small polydisperse circular (spc) DNA, was studied by restriction mapping and base sequence analysis. Restriction mapping revealed each clone to be composed solely of imperfect tandem repeats of ca. 170 bp....