In this fundamental study, streptomycin was extracted successfully from urine and plasma using electromembrane extraction (EME). Streptomycin is an aminoglycoside with log P -7.6 selected as extremely polar model analyte. EME a microextraction technique, where charged analytes are under the influence of electrical field, sample, through supported liquid membrane (SLM), into acceptor solution. T...

Prurigo nodularis (PN) is an intensely pruritic, chronic inflammatory skin disease with diffusely distributed hyperkeratotic nodules featuring cutaneous and systemic inflammation. This study investigated the anti-inflammatory properties of nemolizumab, a humanized anti-IL-31RA antibody, by using unbiased plasma proteomics isobaric Tandem Mass Tags® (TMT®) approach to enrich skin-specific protei...

Using of phytobiotics as fish feed additives have been interestingly increased during the recent decade. The aim of the current study was to evaluate the effect of individual and combined level of thyme and vitamin E in common carp. Juveniles were separately fed by four distinct diets including control (commercial diet without any additive), supplemented with 1% ground Shirazi thyme, supplement...

Background: Normal tissue toxicity continues to remain as a major challenge for radiation oncologists for delivering the total dose to the tumour cells in cancer patients. Cellular, molecular and plasma based early biomarkers to predict the overreactions and non-overreactions of normal tissue toxicity before the initiation of radiotherapy can be valuable for personalised treatment. The aim of t...

Tumor-derived, circulating proteins are potentially useful as biomarkers for detection of cancer, for monitoring of disease progression, regression and recurrence, and for assessment of therapeutic response. Here we interrogated how a protein's stability, cellular localization, and abundance affect its observability in blood by mass-spectrometry-based proteomics techniques. We performed proteom...

Plasma proteins are the most important proteins of human body and include enzymes, regulatory proteins, clotting factors and hormones. They perform diverse functions in the human body and play a cardinal role in its normal functioning. An aberration in structure, function or concentration of even one plasma protein may have fatal consequences. As a result, the study of plasma proteins becomes i...

 The Indian riverine buffaloes are more susceptible to variations in the environment. These variations affect seminal plasma proteins, which in turn affect fertility. This study was conducted to evaluate variations in the Indian Bubalus bubalis seminal plasma protein profile using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) during winter and summer seasons, and its relation wit...

Background: Plasmapheresis is a well-recognised method for harvestingplasma.  It was introduced in the 1950s and is currently employed worldwide.  This method has been in use in Iran since 1979. Objective: To evaluate the efficacy of donor safety program used at the Iranian Blood Transfusion Organization. Methods: A comparison is made of the protein concentrations between the first-time and lon...

objective(s)the aim of this study was to evaluate plasma levels of nitric oxide (no) and certain acute phase proteins(caeruloplasmin, transferrin, haptoglobin, c-reactive protein) in nigerian subjects after short and prolonged exercises.materials and methodsa total of 57 subjects (34 males and 23 females) between ages of 19 and 45 years participated in this study and weredivided into three grou...