CD44 is the major cell surface receptor for the extracellular matrix glycosaminoglycan hyaluronan and is implicated in a variety of biological events that include embryonic morphogenesis, lymphocyte recirculation, inflammation, and tumor metastasis. CD44 delivers activation signals to T lymphocytes, B lymphocytes, natural killer cells, polymorphonuclear leukocytes, and macrophages by stimulatin...

Lipid rafts are chemically distinct compartments of the plasma membrane. Their integrity is a prerequisite for vital cellular functions particularly for signalling and trafficking. Their perturbation is associated with development of a broad spectrum of diseases. Lipid rafts are also important for therapeutic effects of some drugs. Moreover, some of the raft associated molecules are useful immu...

Caspase activation, exposure of phosphatidylserine (PS) on the outer surface of the plasma membrane, and rapid phagocytic removal of dying cells are key features of apoptosis. Nonapoptotic/necrotic modes of death occur independent of caspase activation, but the role of phagocytosis is largely unknown. To address this issue, we studied phagocytosis by human monocyte-derived macrophages (HMDM) an...

Boar sperm viability post-thaw differs depending on the ejaculate fraction used, with spermatozoa present in the first 10 mL of the sperm-rich fraction (SRF) (portion 1, P1, sperm-peak portion) displaying the best cryosurvival in vitro compared with that of spermatozoa from the rest of the ejaculate (portion 2 of the SRF plus the post-spermatic fraction), even when using simplified freezing rou...

Staphylococcus aureus is a bacterium that causes various diseases in humans. Cold plasma found to be an alternative eliminate S. aureus. Most studies of cold on mainly focus the physiochemical changes cells. So far, biological responses treatment under different durations have not yet been evaluated. In this study, results showed was effective eliminating At initial exposure (1 min), treated ce...

During cryopreservation sperm encounter oxidative stress due to higher production of ROS molecules and insufficient natural antioxidant defence system. Therefore, present study was designed identify the effects various glutathione (GSH) concentrations on Indian red jungle fowl (Gallus gallus murghi) quality fertility pre-freezing post-thaw incubation hours. Semen collected from eight cocks qual...

This study examines the relationship between seasonal changes in plasma testosterone and thyroxine concentrations these sperm resistance to freezing-thawing process Merino x Precoz crossbreed rams Florida breed bucks. Plasma concentration, head area, variables their cryoresistance ratios were analyzed throughout a year. Both species showed rise preceding fall. higher during autumn than spring s...

The unexpected enhancement of alkalinee phosphatase (ALP) activity in the serum of a group of STZ-diabetic rats recieving the Teucrium polium extract (with hypoglycaemic property) may be attributed to its probable side effects. In order to get a more precise knowledge about this subject, we evaluated the effect of T. polium extract on the plasma membrane ALP activity of K562 cell line. Our data...

The aim of the project was to use current simple and practical laboratory tests and compare results with the foaling rates of mares inseminated with commercially produced frozen semen. In Exp. 1, semen was tested from 27 and in Exp. 2 from 23 stallions; 19 stallions participated in both experiments. The mean number of mares per stallion in both experiments was 37 (min. 7, max. 121). Sperm morph...

A preceding hypoxic insult can sensitize the cells or the organism to a subsequent, second insult. The aim of the present study was to investigate the molecular mechanism of this phenomenon (often termed 'two-hit' injury paradigm), in an in vitro model of hypoxia/oxidative stress injury in A549 epithelial cells, with special emphasis on the role of the nuclear enzyme poly(ADP-ribose) polymerase...