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background: numerous in vitro reports suggest that low level laser therapy (lllt) affects cellular processes by biostimulation, however most of them emphasize on using visible light lasers which have low penetration. the aim of this study was to determine the effect of infrared laser light (which is more useful in clinic because of its higher penetration) on secretion of fibroblast growth facto...

in this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.then, the cells were cultivated as monolayer in mem (minimum essential medium eagle). the culture mediawere collected after 72 h and frozen at -20°c. carp ovarian follicles also were separated mechanically andincubated in bss (basic salt solution) cortland medium in 24-well microplates for 48 h...

The production of recombinant proteins in moss bioreactors provides all of the benefits of molecular farming in plants but avoids many plant-specific disadvantages, such as the genetic instability of de-differentiated cells in suspension culture or the lack of containment during field production. Protein yields are in the same range as those of other cell-culture-based production systems. On to...

One of the most well-characterised plant pathogenic interactions involves Arabidopsis thaliana and the bacteria Pseudomonas syringae pathovar tomato (Pst). The standard Pst inoculation procedure involves infiltration of large populations of bacteria into plant leaves which means that metabolite changes cannot be readily assigned to the host or pathogen. A plant cell-pathogen co-culture based ap...

INTRODUCTION Cell culture and molecular technologies are basic yet sophisticated research tool used to investigate plant-based medicine for wound healing. METHODS Cell viability and proliferation assay is used to determine whether there are any positive effects and to discover what is the limiting cytotoxic concentration in vitro. The scratch technique, fibroblast-populated collagen lattices ...

Cell suspension cultures are now recognized as important model materials for plant bioscience and biotechnology. Very few studies of metabolic comparisons between cell cultures and original plants have been reported, even though the biological identity of cultured cells with the normally grown plant is of great importance. In this study, a comparison of the metabolome for primary metabolites ex...

Tissue culture is useful method for large scale production of healthy corms and also avenues for creating variability through in vitro use of mutagens and polyploidizing agents. Induction of callus and subsequent regeneration of plants is suggested as possible means of introducing new variation. In vitro micropopagation for product formation its utility is increasing the amount of crocin, picro...

/ 11 Abstract / 12 / 13 One prerequisite for plant functional genomic projects is the development of a high-throughput transformation platform. As the / 14 moss Physcomitrella patens can be transformed via PEG-mediated DNA-uptake into protoplasts, a semi-continuous bioreactor / 15 culture of this plant was optimised regarding protoplast isolation efficiencies. Under standard conditions protopla...