Public key cryptography can uniquely enable trust within distributed settings. Employing it usually requires deploying a set of tools and services collectively known as a Public Key Infrastructure (PKI). PKIs have become a central asset for many organizations, due to distributed IT and users. Even though the usage of PKIs in closed and controlled environments is quite common, interoperability a...

We have initiated an analysis of protein kinase A (PKA) in Drosophila using transgenic techniques to modulate PKA activity in specific tissues during development. We have constructed GAL4/UAS-regulated transgenes in active and mutant forms that encode PKAc, the catalytic subunit of PKA, and PKI(1-31), a competitive inhibitor of PKAc. We present evidence that the wild-type transgenes are active ...

Muscarinic agonist-induced contraction of the ciliary muscle is generally believed to increase aqueous outflow facility and effect accommodation. We used cultured human ciliary muscle cells as a model to study the muscarinic receptor subtype(s) involved in the contractile response of the muscle. Thus, a single cell contraction assay for these muscle cells was developed. And since agonist-induce...

It has been conventional wisdom that, for e-commerce to fulfil its potential, each party to a transaction must be confident about the identity of the others. Digital signature technology, based on public key cryptography, has been claimed as appropriate means to achieve this aim. Digital signatures do little, however, unless a substantial 'public key infrastructure' (PKI) is in place, such that...

In the current rocket-speed growing E-commerce market, certain infrastructure, which enables users to exchange information and money securely over the Internet, is essential. Unfortunately, Internet is mere a cloud of connections of nodes. There is no organization to operate and manage. So the Internet itself is not responsible for any malicious frauds and attacks, and failures caused by reliab...

Public Key Infrastructures (PKIs) are currently being deployed in increasing sizes, numbers, fast changing technologies, and varying environments but our operational experience to date has been limited to a relatively small scale and small number of environments. Consequently, some open technical and environmental interoperability problems about the ways in which PKIs will be organized and oper...

A PKI in support of secure Internet routing was first proposed in [1] and refined in later papers, e.g., [2]. In this “Resource” PKI (RPKI) the resources managed are IP address allocations and Autonomous System number assignments. In a typical PKI the validation problem for each relying party is fairly simple in principle, and is well defined in the standards, e.g. RFC 3280 [3]. The RPKI presen...

Based on a known pharmacophore model for 5-HT₆ receptor antagonists, a series of novel extended derivatives of the N-arylsulfonyindole scaffold were designed and identified as a new class of 5-HT₆ receptor modulators. Eight of the compounds exhibited moderate to high binding affinities and displayed antagonist profile in 5-HT₆ receptor functional assays. Compounds 2-(4-(2-methoxyphenyl)piperazi...

Midline signaling by Hedgehog (Hh) family members has been implicated in patterning the vertebrate embryo. We have explored the potential regulatory role of cAMP-dependent protein kinase A (PKA) in these events. Zebrafish embryos injected with RNAs encoding Sonic hedgehog (Shh), Indian hedgehog (Ihh), or a dominant-negative regulatory subunit of PKA, PKI, have equivalent phenotypes. These inclu...

Dieser Artikel liefert einen Beitrag für die Innovationsforschung bzgl. der Digitalen Signatur und deren Einsatz in Geschäftsprozessen. Die Digitale Signatur wird allgemein als die Grundlage für verbindliche Authentifizierung und sichere Transaktionen in der modernen Internetökonomie angesehen. Sie ermöglicht den beweiswürdigen Ersatz der eigenhändigen Unterschrift. Für den Einsatz einer beweis...