We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the K(D) value of aptamer-protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer-target protein interactions during aptamer selection.

This study developed surface finishing methods to prevent corrosion of electropolished SUS316L stainless steel in isopropyl alcohol（IPA）. For this development, immersion tests 99.7% IPA and anodic polarization 0.15 kmol･m－3 HCl were conducted using specimens passivated with 30% HNO3, 0.1 Na2MoO4-containing 3.5% H2O2 0.98% H2O2- containing 8% citric acid. Identical also applied heated 200－450 ℃ ...

OBJECTIVE To compare therapeutic feeding programme coverage for severely malnourished children achieved by a community-based therapeutic care (CTC) programme and a therapeutic feeding centre (TFC) programme operating in neighbouring districts in Malawi. DESIGN Two surveys were implemented simultaneously one in each of the two programme areas. Each survey used a stratified design with strata d...

Hereditary angioedema (HAE) is a rare autosomal dominant disease that usually occurs in adolescence and early adulthood. It is characterized by recurrent non-pitting edema involving the skin and intestinal tract, especially the extremities and face. It is not associated with urticaria and pruritus. The cause is known to be the deficiency of C1 inhibitor. We herein report a 7-year-old girl with ...

A flow loop has been setup to allow simulation of operating conditions in a steam turbine during transitions water chemistry between on-load (deaerated) and off-load (aerated) environments. Electrochemical noise measurements were performed establish the critical chloride concentration for pitting 403 12Cr stainless steel. The results confirmed by comparative corrosion potential visual observati...