An NADP(+)-dependent D-xylose dehydrogenase from pig liver cytosol was purified about 2000-fold to apparent homogeneity with a yield of 15% and specific activity of 6 units/mg of protein. An Mr value of 62,000 was obtained by gel filtration. PAGE in the presence of SDS gave an Mr value of 32,000, suggesting that the native enzyme is a dimer of similar or identical subunits. D-Xylose, D-ribose, ...

The mechanism of phosphoryl-group transfer from phosphoenolpyruvate (PEP) to HPr, catalyzed by enzyme I of the Escherichia coli PEP-dependent phosphotransferase system, has been studied in vitro. Steady-state kinetics and isotope exchange measurements revealed that this reaction cannot be described by a classical ping-pong mechanism although phosphoenzyme I acts as an intermediate. The kinetic ...

It has become clear as the result of the pioneering studies of Northrop on transcarboxylase (l), and the later studies in Lardy’s (2) and Utter’s (3) laboratories on pyruvate carboxylase that biotin-containing enzymes have two-site ping-pong mechanisms in which biotin is carboxylated at one site, and in turn carboxylates a substrate at the second site. The rate equations for such mechanisms hav...

The crucial issue of quantum communication protocol is its security. A quantum communication scheme must be based on the existing or nearly existing technology, but its security must be guaranteed against an eavesdropper whose technology is limited only by the laws of quantum mechanics. In this paper, we show that all the deterministic and direct two-way quantum communication protocols, sometim...

Both supraventricular or ventricular mechanisms of bidirectional VT (BVT) have been proposed in the past, involving either focal or reentrant mechanisms.(Levy 1989) A supraventricular mechanism with alternating LAFB and LPFB(Rosenbaum 1969)was largely excluded with the advent of intracardiac recordings, which failed to show a His bundle potential preceding the alternating QRS complexes during B...

Introducation. The activities of physical culture and sport subjects are related to the issues popularization certain sports among population. result development game should be seen in quantitative qualitative achievements indicators involvement groups population one or another sport. Aim: establish dynamics basic Ivano-Frankivsk region during 2019−2021. Material methods. Theoretical analysis g...