An alternative method for detecting the production of slime by coagulase negative staphylococci was compared with the routinely used Christensen method on 124 isolates of coagulase negative staphylococci from carriage sites, blood cultures, and infected peritoneal dialysis fluids. The alternative method requires the use of a specially prepared solid medium--brain heart infusion broth, supplemen...

Amyloidosis represents a heterogenous group of diseases that have in common the deposition of fibrils composed of proteins of beta-pleated sheet structure, a structure which can be specifically identified by histochemistry using the Congo red or similar stains. Amyloid consists primarily of the amyloid fibrils but also of the amyloid P component (AP). This component, which is identical with the...

Examination with the light microscope and any of several differential stains has failed to resolve the exact significance of the so-called initial bodies and plaque forms which, together with the elementary bodies, represent stages in the developmental cycles of the Chlamydozoaceae (lymphogranuloma-psittacosis group of agents; Rake, 1947). Previous studies from this laboratory (Rake and Jones, ...

Inhalation of fluorocarbon polymers can cause pulmonary toxicity. Although multiple cases of lung injury have been reported, cellular characterization of the associated alveolitis occurring acutely after inhalation is limited. We report the case of a previously healthy woman who presented at our Emergency Department with an acute pneumonitis following inhalation of a fluorocarbon polymer-based ...

objective the aim of this study was to compare the sensitivity and specificity of acid fast and auramine-rhodamine staining and multiplex pcr for the detection of mycobacterium tuberculosiscomplex and non tuberculosis mycobacteria on formalin fixed paraffin embedded tissues (ffpe) materials and methods forty cases of ffpe pleural and bronchial tissue with chronic granulomatous inflammation and ...

A wound represents the interruption of the continuity of tissue that is followed by damage or cellular death. Wound healing occurs due to a competitive mechanism between the synthesis and lysis of collagen. Any factor that increases collagen lysis or reduces its synthesis may result in changes in the healing process, i.e., nutritional deficiencies. Phototherapies have been suggested as an effec...

We present a large comparative series of trypan blue use in cataract surgery. This series of trypan blue used in all eyes regardless of cataract severity may be unique. We found an apparent increased rate of cystoid macular oedema (CMO) associated with trypan blue use. Melles et al’s report on the use of trypan blue in cataract extraction in 1999 combined with Apple et al’s series on dye enhanc...

The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organisms were rendered green fluorescent by SYTO 13; conversely, gram-positive organisms were rendere...

Benign cephalic histiocytosis (BCH) is a rare type of non-Langerhans histiocytosis with features of many small yellow, yellow-red, or yellow-brown papules located on face, head, neck, and shoulders of infants and young children. There would be no associated symptoms. The histopathological images reveal infiltration of histiocytes in dermis; immunohistochemically, the histiocytes are positive fo...

Crude ethanolic extract and column chromatographic fractions of the Allepey cultivar of Curcuma longa Roxb, commonly called turmeric (tumeric) in commerce, were used as a stain for tissue sections. Staining was carried out under basic, acidic and neutral media conditions. Inorganic and organic dissolution solvents were used. The stain was used as a counterstain after alum and iron haematoxylins...