We report a mechanism-based screening technique to rapidly identify eukaryotic topoisomerase I targeting agents. The method is based on genetic tagging of topoisomerase I to immobilize the enzyme on a solid surface in a microtiter well format. DNA is added to the wells, and retained DNA is detected by Pico Green fluorescence. Compounds that result in an increase in Pico Green staining represent...

A well-formulated research question should incorporate the components of a ‘problem’, an ‘intervention’, ‘control’, and ‘outcome’—at least according to PICO mnemonic. The utility this format, however, has been said be limited clinical studies that pose ‘which’ questions demanding correlational study designs. In contrast, its suitability for descriptive approaches outside investigations doubted....

Background: Super oxidized water (SOW), as a novel antiseptic solution, is used with claims of effectiveness and cost effectiveness in healing chronic wounds such as diabetic foot, infectious post-operative ulcers and burn ulcers. We conducted a health technology assessment to evaluate the clinical evidence from clinical and randomized trials for this disinfection. This study aims to evaluate t...

An integrated sensor system is presented which displays passive long range operation to 100 km at pico-strain (pepsilon) sensitivity to low frequencies (4 Hz) in wavelength division multiplexed operation with negligible cross-talk (better than -75 dB). This has been achieved by pre-stabilizing and multiplexing all interrogation lasers for the sensor array to a single optical frequency reference...

RNA samples and ladders were prepared according to the protocols described in the instruction manuals for the Experion RNA analysis kits and the Agilent 6000 LabChip kits. RNA samples and ladders were first heat-denatured for 2 min at 70°C and then kept on ice until use. The heat-denatured total RNA and mRNA stocks were diluted to the desired final concentrations in either RNase-free TE buffer,...