A study was conducted to characterize aspects of the immune system in the pig from 1 to 30 d of age. Pigs were killed on d 1 (n = 6), d 18 or 19 (n = 6), and d 27 to 30 (n = 7) of age. Lymphocytes were isolated from the blood, thymus, and spleen. Lymphocyte function was assessed for ability to proliferate as induced by concanavalin A (Con A), phytohemagglutinin (PHA), and pokeweed mitogen (PWM)...

The technique of flow cytofluorometry has been employed to assess the response of unfractionated and highly purified human lymphocyte subpopulations to phytohemagglutinin (PHA) and pokeweed mitogen. Normal values for cytofluorometric responses were established and compared to the uptake of tritiated thymidine in simultaneous experiments. Cytofluorometric analysis offered the advantages of incre...

Chromosome analysis, after bromodeoxyuridine incorporation and a sequential Leishman acridine orange staining method previously described, was used to assess the percentage of early or active fragile Xs compared with the overall total in informative cells in 13 heterozygous females. The percentage thus obtained was then used to calculate the percentage of early or active fragile X that would be...

This experiment was performed utilizing a total of 21 primiparous crossbred sows to determine whether a state of immunological suppression occurs during the latter stages of pregnancy in the pig and whether dichlorvos treatment affects immunological function of sows. Immunological reactivity was monitored by the use of in vitro lymphocyte transformation experiments employing the mitogens phytoh...

Lerman and Iwata (1996) described a method for distinguishing between sensory extinction and punishment effects of response blocking on self-injurious behavior maintained independent of social contingencies. Results of their study suggested that blocking decreased the self-injurious hand mouthing of their participant via punishment. The current replication of these procedures with the self-inju...

Measurements with an atomic force microscope (AFM) offer a direct way to probe elastic properties of lipid bilayer membranes locally: provided the underlying stress-strain relation is known, material parameters such as surface tension or bending rigidity may be deduced. In a recent experiment a pore-spanning membrane was poked with an AFM tip, yielding a linear behavior of the force-indentation...

Ribosome-inactivating proteins, such as the pokeweed antiviral protein (PAP), inhibit translation by depurinating the conserved sarcin/ricin loop of the large ribosomal RNA. Depurinated ribosomes are unable to bind elongation factor 2, and, thus, the translocation step of the elongation cycle is inhibited. Though the consequences of depurination are well characterized, the ribosome conformation...

The responsiveness of murine lymphocytes and human peripheral blood lymphocytes to phytohemagglutinin, concanavalin A, pokeweed mitogen, and endotoxin was tested in vitro at 32, 35, and 37 degrees C. The responses at 32 degrees C were delayed and often depressed. Mouse cells responded equally well at 35 and 37 degrees C. Human lymphocytes often responded more rapidly at 37 than at 35 degrees C....

BACKGROUND This paper reports on the aetiology and outcome of childhood open and closed globe eye injuries presenting to the Children's Hospital at Westmead, Sydney, Australia from January 2000 to December 2008. DESIGN Hospital-based retrospective study. PARTICIPANTS Two hundred three cases of childhood eye injuries identified in a nine-year period, comprising 81 open globe and 122 closed g...

Suppression of cellular immunity during primary and secondary infection may explain, in part, the unusual clinical evolution of syphilis. We have previously shown that lymphocytes from normal subjects undergo blastic transformation when exposed in vitro to Treponema refringens. This response was suppressed in patients with syphilis. the suppression being unrelated to serum factors. In the prese...