A study evaluated whether a coated (C) bacterially-derived, non-intrinsically thermostable, phytase product showed equivalent bioefficacy compared to the uncoated version of the same product (Phyzyme XP; 6-phytase, EC 3.1.3.26). Male Ross 308 chicks were assigned to three dietary treatments each with 6 cage replicates of 8 birds each The treatments were: Control (NC), NC + 500 U/kg phytase and ...

A phytase gene was identified in a publicly available metagenome derived from subsurface groundwater, which was deduced to encode for a protein of the histidine acid phosphatase (HAP) family. The nucleotide sequence of the phytase gene was chemically synthesized and cloned, in order to further overexpress the phytase in Escherichia coli. Purified protein of the recombinant phytase demonstrated ...

A meta-analysis was conducted to determine the effect of microbial phytase in pig diets on digestibility and bioavailability of Cu and Zn. Studies (n = 22) into effects of microbial phytase on digestibility and plasma levels of Cu and Zn were included in a dataset and regression analysis was performed to quantify the effect of Aspergillus niger derived 3-phytase in studies (n = 14) with a maxim...

The effects of phytate and phytase on broiler performance and cellular and humoral immunity was assessed by using 504 Cobb 500 female broilers fed nutritionally marginal diets. At 1 d of age, the chicks were randomly allocated to 6 treatments in a 2 x 3 factorial arrangement including 2 levels of phytate P (0.22 or 0.44%) and 3 dose rates of an Escherichia coli-derived phytase [0, 500, or 1,000...

A 14-d experiment was conducted to study the effects of 2 dietary variables on efficacy of a 6-phytase from Citrobacter braakii on broiler growth performance and tibia mineralization. Diets were formulated with or without nutrient matrix values for phytase as negative or positive control (NC or PC, respectively) and with 2 Ca:total P (tP; 2:1 or 2.5:1). The diets were supplemented with 0, 1,000...

Since the mono gastric animals are not capable of metabolizing phytate, a rich source of Phosphorus, undigested phytate is excreted in manure without absorbed for metabolism and poses serious phosphorus pollution in the environment. Therefore, supplementation of phytases into animal feed is expected to solve the above problems. The present investigation deals with cloning and expression of a ph...

Improving the efficiency of P use by pigs is especially important for lactating sows, whose metabolic requirements for P and Ca are high. The effect of a sp. phytase on lactating sow performance and nutrient digestibility was investigated using the combined data set for 6 studies. Treatments included a nutritionally adequate positive control diet (PC), a negative control diet (NC; with an avera...

A 14-d broiler experiment was conducted to assess the effects of 2 dietary variables on efficacy of a bacterial 6-phytase from Citobacter braakii on nutrient and phytate P (PP) utilization. Diets were formulated with or without nutrient matrix values (matrix) for phytase as negative control (NC) or positive control (PC), respectively, and with 2 Ca:total P (tP) levels (2:1 or 2.5:1). The diets ...

The antinutritional property of phytate is the main problem associated with food animals, but phytase-producing probiotic strain could be best alternative for resolving this problem. In present study, phytase from Bacillus subtilis P6 was purified to homogeneity, which showed a relative molecular mass 40 kDa on native and SDS-PAGE. purification fold 86.99, specific activity 104.39 U/mg protein....