We tested the hypothesis that increased systemic vascular resistance in spontaneously hypertensive rats may be secondary to enhanced phospholipase C activity in response to vasoconstrictor stimuli. Activation of phospholipase C by angiotensin II (Ang II), thromboxane A2, arginine vasopressin, and endothelin-1 was compared in cultured glomerular mesangial cells and mesenteric vascular smooth mus...

TRPC6 is a non–voltage-gated Ca entry/depolarization channel associated with vascular tone regulation and remodeling. Expressed TRPC6 channel responds to both neurohormonal and mechanical stimuli, the mechanism for which remains controversial. In this study, we examined the possible interactions of receptor and mechanical stimulations in activating this channel using the patch clamp technique. ...

NMDA receptor-induced excitotoxicity has been hypothesized to mediate abnormal choline (Cho) metabolism that is involved in alterations in membrane permeability and cell death in certain neurodegenerative disorders. To determine whether NMDA receptor overactivation modulates choline metabolism in vivo, we investigated the effects of NMDA on interstitial choline concentrations using microdialysi...

Oil bodies (OBs) are lipid-storing organelles of soybean seeds and the source substrates for enzymatic oxidation reaction soymilk flavour formation. However, triacylglycerols (TAGs) in OBs surrounded by a membrane composed phospholipids embedded with integral oleosins to prevent them from being hydrolyzed lipase. Therefore, specific process formation becomes complicated is not well understood. ...

We constructed the significant high expression (fold change ≥ 2) cytosolic iron-sulfur protein assembly 1 (CIAO1) downstream activation of phospholipase A2 and hormone-mediated signaling-induced cell death network in human Hepato Cellular Carcinoma (HCC), compared with low expression no-tumor hepatitis/cirrhotic tissues (HBV or HCV infection) in GEO data set, by using integration of gene regula...

H2O2 stimulates gallbladder muscle contraction and scavengers of free radicals through the generation of PGE2. Oxidative stress causes lipid peroxidation and generation of platelet-activating factor (PAF) or PAF-like lipids. The present studies therefore were aimed at determining whether either one induced by H2O2 mediates the increased generation of PGE2. Dissociated muscle cells of guinea pig...

The infection of HeLa cells by poliovirus leads to profound alterations in the activities of both phospholipase C and the A23187-stimulated phospholipase A2. As early as the third hour after poliovirus infection, the activity of phospholipase C is enhanced, as measured by the increase in inositol triphosphate (IP3) in the cells. By the fifth hour post-infection there is a 5-fold increase in IP3...

A photoreactive derivative of phosphatidylethanolamine, N-(4-azidobenzoyl)phosphatidylethanolamine (AB-PE), was synthesized by acylation of phosphatidylethanolamine with an N-hydroxysuccinimide ester of 4-azidobenzoic acid. The substantial photosensitivity exhibited by AB-PE correlated with a marked decrease in the absorption spectra of the compound. The compound proved sensitive to lipase and ...

Abstract Nephrotic syndrome (NS), which includes primary and secondary types, is one of the causes end-stage kidney disease. Common in adults include diabetic nephropathy (DN), membranous (MN), focal segmental glomerulosclerosis. About 30%–40% diabetics can develop into DN. The incidence nephrotic patients was not significantly different from that general population. Herein we present a windfal...