New analytical methods have been developed and validated on high performance liquid chromatography (HPLC) to assess the assay, content uniformity and dissolution of immediate release candesartan cilexetil 32 mg tablets. Method development studies were performed on cyano column. Mobile phase of assay and content uniformity test consisted of mixture of 0.05 M phosphate buffer, pH 4.5 and methanol...

A diluted solution of a mixture of Hb S and Hb A in concentrated phosphate buffer was found to aggregate with a clear demonstration of delay time. The mechanism of the participation of Hb A in the aggregation of deoxy Hb S in concentrated phosphate buffer has been investigated. Both the delay time and the aggregation time in the polymerization reaction of mixtures of Hb A and Hb S were prolonge...

The liver and kidneys were removed aseptically from non-fasted adult male rabbits, weighing 2.7 to 4.5 kilos, which were anesthetized with Dialurethane. The homogenate’ of the tissues was prepared by mixing the tissue, testosterone, and 50 ml. of the Ringer’s phosphate buffer in the Waring blendor for 2 minutes. The mixture was transferred to a Fernbath flask with another 50 ml. of buffer and i...

The testing for drugs of abuse in hair is increasingly used to detect illicit substances. Laboratories have implemented various decontamination, or washing, procedures in order to eliminate concerns regarding potential contamination of the hair with drug from the environment. However, the effect of these decontamination procedures on drug incorporated into the hair shaft via systemic exposure i...

1. No significant change with time (to 24 hours) in the cataphoretic velocity of certain mammalian red cells occurs when the cells are suspended in M/15 phosphate buffer at pH = 7.35. Neither successive washings nor standing effect a change. 2. In M/15 phosphate buffer at pH 7.35 +/- 0.03 the following order of red cell velocity has been obtained. The numbers in parenthesis are micro per second...

The aim of this study was to evaluate the behavior of high-noble gold-platinum alloy samples (18 + 8 Rafinerija plemenitih kovina, Zagreb, Hrvatska), in a phosphate buffer pH 6.0. The aim of the study was to determine the type and amount of released metal ions from the alloy, and to evaluate how the time of exposure to the phosphate buffer pH 6.0 influences the release of metal ions from the al...

Abbreviations: Bio-LC-ATB-BMPA, 4,4-O-[2-[ 2-[2-[2-[2-[6-(biotinylamino)hexanolyl]amino]ethoxy]exoxy]ethoxy]-4-(1-azi2,2,2-trifluoroethyl)benzoyl]amino-1,3-propanediyl]bis-D-mannose; HES, HEPES, EDTA sucrose; KRH buffer, Krebs-Ringer Hepes buffer; KHB, Krebs-Henseleit bicarbonate; PBS, phosphate-buffered saline; TBS-T, Tris-buffered saline, 0.1% Tween; Carboxy-SNARF-1, 5-(and 6-)carboxy-semi-na...

Reversed phase high pressure liquid chromatography (HPLC) solvent systems have been developed for the separation of intermediates in the formation of bile acids and bile acid conjugates from cholesterol. Four different mobile phases (water-methanol, 10 mM acetate buffer (pH 4.37)-methanol, 30 mM trifluoroacetic acid (pH 2.9 with triethylamine)-methanol, and 50 mM potassium phosphate buffer (pH ...

Preparation of Enzyme SolutionsRabbit muscle glyceraldehyde-3-phosphate dehydrogenase was prepared as described previously (1) and stored at 0” as the crystalline suspension. For use, the crystals were separated by centrifugation and dissolved in buffer. The resulting solution was dialyzed overnight against a flowing stream of buffer in order to remove the ammonium sulfate, equilibrated against...

Previous studies by us and others have demonstrated that PGE2 and thromboxane (Tx) B2 are produced in the fetal and neonatal brain during cerebral hypoperfusion. The present study was to test the hypotheses that indomethacin would alter the cerebral blood flow (CBF) response to reduced cerebral perfusion pressure in late-gestation fetal sheep by inhibiting the local prostanoid production. We st...