نتایج جستجو برای: phenylalanine ammonia lyase pal

تعداد نتایج: 53088  

Journal: :International journal of biological sciences 2016
Ningbo Wang Mengjie Liu Lihua Guo Xiufen Yang Dewen Qiu

Here we reported a novel protein elicitor from Bacillus amyloliquefaciens NC6 induced systemic resistance (ISR) in tobacco. The purification was executed by ion-exchange chromatography, native-page extraction and HPLC, and the amino acid sequence was identified by mass spectrometry. This recombinant elicitor protein, expressed in Escherichia coli by an E1 expression vector, had good thermal sta...

2013
Joachim Bollmann Klaus Hahlbrock

The timing o f changes in the protein synthesis pattern o f elicitor-treated, [35S]methioninelabelled parsley cells ( Petroselinum crispum) was analyzed by two-dimensional gel electropho­ resis. Five groups were distinguished from a large number o f elicitor-responsive as well as un­ responsive proteins. Two groups were synthesized de novo either early or late after elicitor ap­ plication; two ...

Journal: :Indian journal of experimental biology 2004
Miral Patel I L Kothari J S S Mohan

Perception of microbial signal molecules is part of the strategy evolved by plants to survive attacks by potential pathogens. To gain a more complete understanding of the early signaling events involved in these responses, we used fungal components of Fusarium under in vitro condition and checked the rise in signal molecule, salicylic acid (SA), and marker enzymes in defense reactions against t...

2015
Nilanjan Chakraborty Swarnendu Chandra Krishnendu Acharya

Effect of sublethal heavy metal stress as plant biotic elicitor for triggering innate immunity in tomato plant was investigated. Copper in in vivo condition induced accumulation of defense enzymes like peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), and β-1,3 glucanase along with higher accumulation of total phenol, antioxidative enzymes (catalase and ascorbate per...

2014
Jiang-Lian Duan Xiao-Li Ma Guang-Tao Meng Jian-Guo Xu

In this study, changes in some chemical compositions associated with fruit quality and enzymatic activities were investigated during ripening of hawthorn fruits from the 88th to 148th day after full bloom day (from August 11 to October 10, 2013). Significant differences in these indices were found between different maturation stages as well as hawthorn cultivars. During ripening of hawthorn fru...

2013
Andrea Goldson-Barnaby Christine H. Scaman

Trichosporon cutaneum phenylalanine ammonia lyase was selected as a model to investigate the dual substrate activity of this family of enzymes. Sequencing of the PAL gene identified an extensive intron region at the N-terminus. Five amino acid residues differing from a prior report were identified. Highest Phe : Tyr activities (1.6  ± 0.3 : 0.4 ± 0.1  μ mol/h g wet weight) were induced by Tyr. ...

2005
Young-Jun Choi Francisco A. Tomás-Barberán Mikal E. Saltveit

A wound signal originates at the site of injury in lettuce (Lactuca sativa L.) leaf tissue and propagates into adjacent tissue where it induces a number of physiological responses which include increased phenolic metabolism with the de novo synthesis of phenylalanine ammonia lyase (PAL, EC 4.3.1.5), the synthesis and accumulation of soluble phenolic compounds (e.g., chlorogenic acid), and subse...

2005
F. Broetto

Plant cells are able to shift their metabolism in response to aggressive environmental factors such as salinity, water stress, anoxia, flooding or biotic elicitors. In this work, cell suspensions of four bean (Phaseolus vulgaris L.) genotypes were stabilized and treated with elicitors isolated from fungal (Fusarium oxysporum f. sp. phaseoli) cell walls. The elicitation of the cells was effectiv...

Journal: :The Biochemical journal 1998
R Desikan A Reynolds J T Hancock S J Neill

Programmed cell death is increasingly viewed as a key component of the hypersensitive disease resistance response of plants. The generation of reactive oxygen species (ROS) such as H2O2 triggers a cell death programme in Arabidopsis suspension cultures following challenge with the bacterial elicitor harpin. Both harpin and exogenous H2O2 initiate a cell death pathway that requires gene expressi...

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