In this paper, the extraction of polyphenols from amaranth seed using a Box–Benhken design four factors—ultra-turrax speed, solid-to-liquid ratio (RSL), methanol concentration and time—were studied. There were two responses studied for model: total phenolic content (TPC) flavonoid (TFC). The factors which influenced most TPC TFC RSL, ultra-turrax speed. Twelve acids (rosmarinic acid, p-coumaric...

A rapid procedure for measuring the specific radioactivity of phenylalanine in tissues was developed. This facilitates the accurate determination of rates of protein synthesis in a wide range of tissues by injection of 150 mumol of L-[4-(3)H]phenylalanine/100 g body wt. The large dose of amino acid results in a rapid rise in specific radioactivity of free phenylalanine in tissues to values clos...

Volume 5 • Issue 6 • 1000e125 J Genet Syndr Gene Ther ISSN: 2157-7412 JGSGT, an open access journal Hyperphenylalaninemias (HPAs; OMIM 261600) are related to phenylalanine hydroxylase (PAH; OMIM *612349) deficiency; a hepatic enzyme, and are characterized by moderate and/or high levels of the amino acid phenylalanine and reduction of tyrosine. High levels of phenylalanine and low of tyrosine ch...

Cerebral malaria is associated with decreased production of nitric oxide and decreased levels of its precursor, l-arginine. Abnormal amino acid metabolism may thus be an important factor in malaria pathogenesis. We sought to determine if other amino acid abnormalities are associated with disease severity in falciparum malaria. Subjects were enrolled in Dar es Salaam, Tanzania (children) (n = 12...

The addition of D-phenylalanine to starved cultures of Neurospora crassa leads to de novo synthesis of L-amino-acid oxidase. Poly(A) RNA from D-phenylalanine-treated mycelium was therefore used to generate a cDNA library which was subsequently screened by hybrid-selected translation. A positive L-amino-acid oxidase clone served as a probe to isolate the complete gene from a genomic library of N...

7-Amino-4-methylcoumarin (AMC) is a low molecular weight fluorescent probe that can be attached to peptide enable the detection of specific proteases, such as chymotrypsin, expressed in certain diseases. Because this depends on specificity protease toward peptidyl AMC, development substrates required. To investigate AMC compounds incorporating four different amino acid residues were prepared by...

A novel enzyme with a specific phenylalanine aminopeptidase activity (ApsC) from Aspergillus niger (CBS 120.49) has been characterized. The derived amino acid sequence is not similar to any previously characterized aminopeptidase sequence but does share similarity with some mammalian acyl-peptide hydrolase sequences. ApsC was found to be most active towards phenylalanine beta-naphthylamide (F-b...

Aromatic amino acid ammonia-lyases and aromatic amino acid 2,3-aminomutases contain the post-translationally formed prosthetic 3,5-dihydro-4-methylidene-5H-imidazol-5-one (MIO) group. MIO enzymes catalyze the stereoselective synthesis of α- or β-amino acid enantiomers, making these chemical processes environmentally friendly and affordable. Characterization of novel inhibitors enables structura...

Phenylacetic acid and p-hydroxyphenylacetic acid are formed upon incubation of photosynthetic membranes from the prokaryotic alga Anacystis nidulans with L-phenylalanine and L-tyrosine, respectively. The corresponding phenylpyruvic acids act as intermediates as shown by trapping them as the stable oximino acids. The first step in this reaction sequence appears to be catalyzed by a thylakoid-bou...

We recently demonstrated that muscle protein synthesis was stimulated to a similar extent in young and elderly subjects during a 3-h amino acid infusion. We sought to determine if a more practical bolus oral ingestion would also produce a similar response in young (34 +/- 4 yr) and elderly (67 +/- 2 yr) individuals. Arteriovenous blood samples and muscle biopsies were obtained during a primed (...