Accurate disease diagnosis, patient stratification and biomarker validation require the analysis of multiple biomarkers. This paper describes cross-reactivity-free multiplexing of enzyme-linked immunosorbent assays (ELISAs) using aqueous two-phase systems (ATPSs) to confine detection antibodies at specific locations in fully aqueous environments. Antibody cross-reactions are eliminated because ...

BACKGROUND AND PURPOSE Previously, implant survival of total shoulder prostheses was reported to be inferior to that of hemiprostheses. However, the use of total prostheses has increased in Norway due to reported good functional results. On this background, we wanted to study implant survival of 4 major shoulder prosthesis types in Norway between 1994 and 2012. PATIENTS AND METHODS The study ...

a novel, simple and more sensitive spectrophotometric procedure has been developed for the determination of brilliant blue fcf and carmoisine in food samples by an aqueous two-phase system (atps). after extraction, the absorbance values were measured at 527 and 632 nm for carmoisine and brilliant blue fcf, respectively. the effects of different parameters such as, polyethylene glycol (type and ...

isotactic poly (1-butene), ipbu-1, was synthesized by using a metallocene catalyst. the thermodynamic phase behavior of polymer–organic solvents systems is very important in every polymer application.  in this paper, the solid–liquid equilibrium of ipbu-1 with different organic solvents (1-heptyne, cyclo octane) was studied by a mathematical model. by considering the experiments temperature-mol...

Microalgae are a valuable source of enzymes and active ingredients due to their biochemical variability, health-beneficial properties, lower production cost viability on an industrial scale. In addition, the growing demand for sustainable products constitutes microalgae as viable resource development range innovative products. present work, proteolytic activity Arthrospira platensis lysate was ...

coriolis mass flow meters are one of the most accurate tools to measure the mass flow in the industry. however, two-phase mode (gas-liquid) may cause severe operating difficulties as well as decreasing certitude in measurement. this paper presents a method based on fuzzy systems to correct the error and improve the reliability of these sensors in the presence of two-phase model fluid. definite ...

Pineapple peel is a potential source for the extraction of beneficial bioactive compounds due to the large amount of waste after processing. The extraction of bromelain from pineapple peel by using aqueous two-phase system (ATPs) and its application in muscle models were investigated. Bromelain predominantly partitions to the polyethylene glycol (PEG) rich top phase. The highest enzyme activity...