An improved production and purification method for Alzheimer's disease related methionine-modified amyloid-β 1-40 and 1-42 peptides is proposed, taking advantage of the formation of inclusion body in Escherichia coli. A Thioflavin-S assay was set-up to evaluate inclusion body formation during growth and optimize culture conditions for amyloid-β peptides production. A simple and fast purificatio...

This contribution investigates the application of a nonlinear model based adaptive predictive control algorithm to a pH neutralization plant. A local linear model network is responsible for the online identification of the plant. The advantage of such a model is that the information needed by the predictive control algorithm to build gradients is directly available. This aspect reduces the comp...

Hypersecretion of gastric acid damages the stomach lining, causing formation peptic ulcers. Mucilage from medicinal plants offers a relaxing and soothing effect to endodermal lining gut has antacid properties, which can protect mucosal acidity. This is first report aimed evaluate physicochemical characteristics, acid-neutralizing, cytotoxicity properties traditionally used aqueous mucilage Aspa...

OBJECTIVE To assess the pH of regular and light cola-based drinks and energy drinks, and examine the acid neutralizing capacity by the addition of artificial saliva. MATERIALS AND METHODS Ten packages of each product purchased locally at different stores were evaluated. The pH was measured by a pH-electrode calibrated in standard buffer solutions. To assess the pH neutralizing capacity, 1 ml/...

General aspects of modeling as an engineering tool are presented in this work. The usefulness of Takagi-Sugeno Fuzzy Inference Systems (TS FIS) as modeling technique are emphasized. Additionally, various modifications of known methodology for identifying such a models and rules configuration are proposed. Finally, two application examples are presented. The first one is a modeling of the Box an...

BACKGROUND A vaccine is needed to control the spread of human immunodeficiency virus type 1 (HIV-1). An in vitro assay that can predict the protection induced by a vaccine would facilitate the development of such a vaccine. A potential candidate would be an assay to quantify neutralization of HIV-1. METHODS AND FINDINGS We have used sera from rhesus macaques that have been immunized with HIV ...

The basis of dynamic data rectification is a dynamic process model. The successful application of the model requires the fulfilling of a number of objectives that are as wide-ranging as the estimation of the process states, process signal denoising and outlier detection and removal. Current approaches to dynamic data rectification include the conjunction of the Extended Kalman filter (EKF) and ...

Active carbons of different origins have been oxidized with H O and (NH ) S O and their oxygen surface complexes 2 2 4 2 2 8 have been characterized by TPD, classical titration following Boehm’s method and by neutralization calorimetry. The net enthalpies of neutralization, determined by immersion calorimetry into NaOH and HCl 2 N lead to 241.161.8 and 21 21 252.362.0 kJ eq for the acidic and b...

This study evaluated the effect of different types of filler in a resin-based pit and fissure sealant on fluoride release, recharge, and lactic acid neutralization. Resin-based sealant was incorporated with 5% w/w of the following fillers: calcium aluminate cement (CAC), synthesized mesoporous silica (SI), a CAC and SI mixture (CAC+SI), glass-ionomer powder (GIC), and acetic acid-treated GIC (G...