We study the operator-valued free Fisher information of random matrices in an operator-valued noncommutative probability space. We obtain a formula for Φ M2(B) (A,A,M2(B), η), where A ∈ M2(B) is a 2 × 2 operator matrix on B, and η is linear operators on M2(B). Then we consider a special setting: A is an operator-valued semicircular matrix with conditional expectation covariance, and find that Φ...