The Rockefeller University Press $30.00 J. Cell Biol. Vol. 207 No. 5 643–656 www.jcb.org/cgi/doi/10.1083/jcb.201404128 JCB 643 Correspondence to Tina Izard: izard{at}scripps.edu Abbreviations used in the paper: CC, correlation coefficient; FA, focal adhesion; MEF, mouse embryonic fibroblast; PC, phosphatidylcholine; PIP2, phosphatidylinositol 4,5-bisphosphate; VBS, vinculin binding site; VH, vi...

We demonstrate that second-harmonic radiation imaging probes are efficient biomarkers for imaging in living tissue. We show that 100 nm and 300 nm BaTiO(3) nanoparticles used as contrast markers could be detected through 50 μm and 120 μm of mouse tail tissue in vitro or in vivo. Experimental results and Monte-Carlo simulations are in good agreement.