Goal. To conduct molecular genetic studies of products swine breeding, in particular, liver, sold the food markets Kharkiv, for presence genomic material porcine circovirus type 2 (CVS2). Methods. Extraction total nucleic acid, particular CVS2 DNA, from liver homogenate, transported to laboratory on ice was performed according standard methods. Detection by polymerase chain reaction (PCR) a for...

Aim: To compare the results of DNA amplification by the polymerase chain reaction (PCR) with immunofluorescence staining for detecting Pneumocystis carin'i in bronchoalveolar lavage specimens taken from symptomatic HIV seropositive patients with suspected P carinii pneumonia (PCP). Methods: Bronchoalveolar lavage specimens were obtained from 28 symptomatic HIV seropositive patients. Specimens w...

AIM To compare the results of DNA amplification by the polymerase chain reaction (PCR) with immunofluorescence staining for detecting Pneumocystis carinii in bronchoalveolar lavage specimens taken from symptomatic HIV seropositive patients with suspected P carinii pneumonia (PCP). METHODS Bronchoalveolar lavage specimens were obtained from 28 symptomatic HIV seropositive patients. Specimens w...

Denaturing polyacrylamide gel electrophoretic analysis of amplified polymorphic short tandem repeat (STR) loci using fluorescent markers is a mainstay of forensic and paternity testing. To reduce the drawback of preparing gels or using expensive precast gels, we have developed a simple and rapid method to reuse gels between 2 and 8 times over a period of several days. Following the initial elec...

A study was carried out to identify the microbial diversity in wastewater from tuna industry, samples were obtained at different points process, analysis using molecular tools such as metagenomics, Electrophoresis technique Denaturation Gradient Gel (DGGE) and Polymerase Chain Reaction (PCR). The sensitivity of these techniques allowed amplification 16S rDNA gene fragments by means specific pri...

introduction: considering the role of hla matching in transplant outcome, the quality of hla-dr typing is clearly an important issue.in recent years serologic methods have been replaced with dna based typing methods.the main objective of this study was to compare hla-dr typing data obtained from existing serologic method with that obtained by the new pcr-ssp method. material and methods: fifty-...

Inter- and intralaboratory inconsistencies in detection rates of Chlamydia pneumoniae in vascular specimens have been demonstrated. In this study, 66 vascular tissue specimens from 66 patients with vascular disease were tested by three PCR assays: a 16S PCR-based reverse line blot (RLB) assay, a single-step PCR, and a nested PCR. Also, we explored the impacts of different DNA polymerase enzymes...

Demand for herbal products has increased because of their purported health benefits and economic value. However, they are susceptible to adulteration, making accurate identification origin essential, especially quality control. In recent decades, DNA-based methods have played a crucial role in the development authentication tools that required good DNA. The manufacturing process involved heatin...