نتایج جستجو برای: pcr mycoplasma

تعداد نتایج: 187962  

2011
Suk-Ju Kim Dong Sup Lee Seung-Ju Lee

PURPOSE Our purpose was to conduct a screening test for urethritis or cervicitis as a sexually transmitted disease (STD) by using multiplex polymerase chain reaction(PCR) and to determine the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Mycoplasma hominis, and Trichomonas vaginalis in asymptomatic people. MATERIALS AND METHODS From...

Journal: :Applied and environmental microbiology 1994
B Pettersson K E Johansson M Uhlén

Automated solid-phase DNA sequencing was used for determination of partial 16S ribosomal DNA sequences of mycoplasmas. The sequence information was used to establish phylogenetic relationships of 11 different mycoplasmas whose 16S rRNA sequences had not been determined earlier. A biotinylated fragment corresponding to positions 344 to 939 in the Escherichia coli sequence was generated by PCR. T...

2010
Andres Pascual Katia Jaton Béatrice Ninet Jacques Bille Gilbert Greub

Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. hominis in three patients with severe extragenital infections.

Journal: :Journal of clinical microbiology 2001
J W Dorigo-Zetsma R P Verkooyen H P van Helden H van der Nat J M van den Bosch

Mycoplasma pneumoniae infection was diagnosed in 18 (12.5%) of 144 adults hospitalized with community-acquired pneumonia. The infection was demonstrated by PCR in 15 patients and by serology, using two methods, in 10 patients. The mean age of the 8 patients with positive M. pneumoniae PCR and negative serology was significantly higher than that of the 10 patients with positive serology.

Journal: :The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases 2007
Letícia Alves Vervloet Christophe Marguet Paulo Augusto Moreira Camargos

This manuscript reviewed the literature on infection by Mycoplasma pneumoniae with emphasis on etiological aspects of childhood community-acquired pneumonias. Bibliographical research was carried out from Pubmed Medline, MDConsult, HighWire, LILACS, and direct research over the past 10 years with the following keywords: Mycoplasma pneumoniae, pneumonia, and childhood. Fifty-four articles were s...

Journal: :The Malaysian journal of pathology 2014
F Jahan S M Shamsuzzaman S Akter

Urethritis is one of the most important causes of morbidity and mortality in developing countries. The aim of this study was to detect common bacterial causes of urethritis in men by Gram stain, culture and multiplex PCR.185 male patients who presented at the Skin and venereal clinic of the Dhaka Medical College, Bangladesh with clinical symptoms suggestive of urethritis were enrolled in this s...

2013
Aline Padilha Fraga Tatiana de Vargas Nilo Ikuta André Salvador Kazantzi Fonseca Álvaro José Celmer Edmundo Kanan Marques Vagner Ricardo Lunge

Mycoplasma gallisepticum (MS) and Mycoplasma synoviae (MS) are important avian pathogens and cause economic losses to the poultry industry. Molecular biology techniques are currently used for a rapid detection of these pathogens and the adoption of control measures of the diseases. The aim of this study was to develop and validate a technique for simultaneous detection of MG and MS by multiplex...

Journal: :Journal of clinical microbiology 2014
Chloé Le Roy Sabine Pereyre Cécile Bébéar

The performance of two commercial real-time PCR kits for the detection of Mycoplasma genitalium was evaluated in comparison to an in-house real-time PCR assay. Concordances of 96% and 93% were found for the TIB MOLBIOL and the Diagenode assays, respectively, compared to the results of the in-house assay.

2016
Fernanda P. Firmino Larissa C. Aquino Tatiana G. Marçola Marta V. Bittencourt Concepta M. McManus Giane R. Paludo

Firmino F.P., Aquino L.C., Marçola T.G., Bittencourt M.V., McManus C.M. & Paludo G.R. 2016. Frequency and hematological alterations of different hemoplasma infections with retrovirusis co-infections in domestic cats from Brazil. Pesquisa Veterinária Brasileira 36(8):731-736. Laboratório de Patologia Clínica, Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Campus Univers...

Journal: :Medycyna Weterynaryjna 2021

The aim of this study was to determine the prevalence hemoplasma species in cats by real-time PCR and distribution species. Furthermore, it aimed evaluate factors that are thought be important epidemiology disease statistically. For purpose, blood samples from 246 were examined for Candidatus Mycoplasma haemominitum (CMhm), haemofelis (Mhf) turicensis (CMt) PCR. CMhm detected 20 (8.13%), CMt 3 ...

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