Mycoplasmas hominis, Mycoplasmas genitalium and Ureaplasma urealyticum are associated with infections of the genitourinary tract, reproductive failure, and neonatal morbidity and mortality. A multiplex PCR was developed for simultaneously detection of these Mycoplasmas species in a single amplification reaction. The total number of 104 samples was collected from 104 women’s genital specimens wi...

Background: Male infertility is a Multifactorial syndrome encompassing a wide variety of disorders. In more than half of infertile men, the cause of their infertility is unknown (idiopathic) and could be congenital or acquired. Globozoospermia, also called round-headed spermatozoa, is a rare disease with incidence< 0.1% among male infertile patients. The most prominent feature of globozoospermi...

introduction: this study was intended to determine the presence and distribution of listeria monocytogenes in various meat and dairy products from qazvin province by culture followed by biochemical and morphological assays. the identity of the isolates was further obtained by amplification of prfa gene in bacteria isolates. this gene is a transcriptional activator of virulence gene expression w...

Avian influenza virus (AIV) can infect a variety of avian species and mammals, leading to severe economic losses in poultry industry and posing a substantial threat to public health. Currently, traditional virus isolation and identification is inadequate for the early diagnosis because of its labor-intensive and time-consuming features. Real-time RT-PCR (RRT-PCR) is an ideal method for the dete...

Anaplasma bovis is a leukocytotropic agent of bovine anaplasmosis and there is no available information about molecular study on this agent in cattle of Iran. In this study a total 150 cattle blood samples were collected from central part of Iran. The presence of A. bovis examined using light microscopic detection and species-specific nested polymerase chain reaction (nested-PCR) based on 16S r...

BACKGROUND Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. METHODS A total of 220 clinical isolates of S. aureus which we...

Urinary tract infections (UTIs) are one of main health problems caused by many microorganisms, including uropathogenic Escherichia coli (UPEC). The virulence factors important in colonization UPEC, extra-intestinal survival, and creation cytopathic effects. In addition, the expression special UPEC can contribute to uropathogenicity, as well worsening UTIs. this study, 134 isolates E. were colle...

The main objective of this study was to develop a reliable detection method for genetically modified cotton events MON 531 and MON 15985 by using multiplex PCR reaction technique. In this study we have used four primer pairs for the detection of individual gene segments present in transgene cassette of two GM cotton events which include CaMv35S promoter, Nos terminator, Cry1Ac and Npt II genes ...