نتایج جستجو برای: pcr and nested
تعداد نتایج: 16854787 فیلتر نتایج به سال:
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetal...
A novel method, which involves a nested PCR in a single closed tube, was developed for the sensitive detection of Erwinia amylovora in plant material. The external and internal primer pairs used had different annealing temperatures and directed the amplification of a specific DNA fragment from plasmid pEA29. The procedure involved two consecutive PCRs, the first of which was performed at a high...
بیماری نکروز عصبی ویروسی، یکی از مهمترین بیماریهای ویروسی با گسترش جهانی در ماهیان دریایی و پرورشی دنیا محسوب میشود و تا به امروز بیش از 70 گونه حساس ماهی به این بیماری شناسایی شده است. هدف از این پژوهش ردیابی و شناسایی سریع ویروس مذکور با استفاده از روش Nested RT-PCR از بافت مغز کفال ماهیان دریای خزر بود. در طول فصل صید در سال 1395 تعداد 40 قطعه کفال طلایی (Liza aurata) در محدوده وزنی 50 ...
BACKGROUND Nosocomial candidaemia is associated with high mortality rates in critically ill paediatric patients; thus, the early detection and identification of the infectious agent is crucial for successful medical intervention. The PCR-based techniques have significantly increased the detection of Candida species in bloodstream infections. In this study, a multiplex nested PCR approach was de...
بیماری پوسیدگی سفید ریشه یکی از مهمترین بیماریهای درختان میوه در دنیا و ایران میباشد که توسط قارچ rosellinia necatrix ایجاد میشود. بهمنظور ردیابی سریع بیمارگر از خاک و ریشه، شش سری خاک از کنار طوقه درختان آلبالو آلوده و دو نمونه خاک به عنوان شاهد منفی و مثبت جمعآوری گردید. استخراج مستقیمdna از هشت سری خاک مذکور و ریشههای آلبالو دارای میسلیومهای سفید و خاکستری روی پوست، ریشههای دارای م...
objective: gbv-c is a virus transmissible via blood transfusion and sexual routes. because of the shared transmission routes in gbv-c and hepatitis b virus (hbv) we have attempted to detect gbv-c in hbsag-positive patients using the more sensitive semi-nested pcr. we used restriction fragment length polymorphism (rflp) to determine genotype. methods: a total of 100 serum samples were collected...
babesiosis is an important tickborne disease in the sheep of iran. objectives: a molecular study was carried out in north khorasan province, iran in 2010-2011, designed to identify babesia spp. infection of both sheep and ticks. methods: ninety sheep from different flocks were clinically examined and blood samples were collected with ixodid ticks . the collected ticks were separated into 82 ti...
background: chlamydia trachomatis (c. trachomatis) and mycoplasma genitalium (m. genitalium) are considered factors in cervical and ovarian cancer and are associated with flaky cell carcinoma of the cervix. the role of steady infection, leading to chronic inflammation, in the of ovarian cancer has received very little consideration, although a background of pelvic inflammatory disease (pid) is ...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید