IT is generally recognized that glycogen is a very labile substance which disappears rapidly from tissues unless treated by special methods of fixation and embedding. The recognized and widely used techniques, advocated by the standard microtomical treatises, call for fixation in a glycogenprecipitating fixative (absolute alcohol; picro-formol-alcohol; picro-dioxane) followed by celloidin embed...

Among the adaptive-grid methods, redistribution and embedding techniques have been the focus of more attention by researchers. Simultaneous or combined adaptive techniques have also been used. This paper describes a combination of adaptive-grid embedding and redistribution methods on semi-structured grids for two-dimensional invisid flows. Since the grid is semi-structured, it is possible to us...

the separation of olefin/paraffin mixtures is an important process in petrochemical industries, which is traditionally performed by low temperature distillation with a high-energy consumption, or complex extractive distillationand adsorption techniques. membrane separation process is emerging as an alternative for traditional separation processes with respect to low energy and simple operation....

in this paper, a faster method for embedding cryptographic information in the image ispresented by expressing the concept of latent prints (steganography). data is encrypted bytwo methods before embedding to increase reliability. then they are embedded into the imageby a button, a method has been expressed to reduce potential noise impact on the wayinformation is encoded.

BACKGROUND The hepatic iron concentration (HIC) is widely used in clinical practice and in research; however, data on the variability of HIC among biopsy sites are limited. One aim of the present study was to determine the variability of HIC within both healthy and cirrhotic livers. METHODS Using colorimetric methods, we determined HIC in multiple large (microtome) and small (biopsy-sized) pa...

UNLABELLED Adrenal cystic lesions are uncommon but due to the improved radiologic imaging techniques their appearance seems to increase. MATERIAL AND METHODS We analyzed the clinical and radiological findings of 10 patients with adrenal cysts and the pathological features of the operative material. Standard dissection procedure and paraffin embedded tissue sections were made, stained by HE an...

METHODS. Protein and mRNA were obtained from human, rabbit, and bovine lens epithelia and from cultured human lens epithelial cells (B3, hLECs) and rabbit lens epithelial cells (N/N1003A, rLECs). Paraffin-embedded sections were prepared from human lenses for immunohistochemical localization of GR. RT-PCR was performed to amplify portions of GR, and the products were sequenced. Protein samples w...

Objectives: To determine the utility of the cell transfer technique (CTT) for the BRAF molecular testing on thyroid fine-needle aspirates (FNA). Methods: Polymerase chain reaction (PCR)based BRAF molecular testing was performed on tissues obtained through CTT from both airdried and ethanol-fixed direct smears of thyroid FNA, and then compared with the corresponding thyroidectomy formalin-fixed,...

Formalin-fixed paraffin-embedded tissues (FFPET) represent the largest source of archival biological material available for genomic studies. In this work we present an advanced protocol for extraction of high quality DNA from FFPET that can be applied in several molecular studies. Although cat mammary tumours (CMT) are the third most frequent tumour in cats the recovery of significant number of...

Laser microdissection (LMD) is an accurate and fast method to procure pure populations of cells from complex, heterogeneous tissues under direct microscopic visualization. It can be applied to a wide range of cell preparation, including paraffin-embedded material. The morphology of the captured cells is retained, and DNA, RNA, and proteins can be extracted for molecular analysis. The potential ...