Putative Pseudomonas spp. isolated predominantly from raw and processed milk were characterized by automated ribotyping and by biochemical reactions. Isolates were biochemically profiled using the Biolog system and API 20 NE and by determining the production of proteases, lipases, and lecithinases for each isolate. Isolates grouped into five coherent clusters, predominated by the species P. put...

Abstract Endophytic bacteria have been reported to roles as biocontrol agents and plant growth promoters. The application of endophytic in the form mixed culture [consortia] will provide better effectiveness than when applied alone. This study aimed obtain a consortium that is effective biological control for purple blotch [ Alternariaporri ], promote yield shallots. research was arranged compl...

Eleven endophytic bacteria were isolated from the roots of cumin, a medicinal plant grown in Assiut Province, Egypt. The strains KAU2025 and KAU2022 exhibited highest inhibition rate against Fusarium oxysporum (FOC7) (64%) dual culture test selected for further studies. identified as Brevibacillus parabrevis Pseudomonas fluorescens, respectively, based on 16S rDNA sequence analysis. isolates in...

Cell yields of Pseudomonas fluorescens, Aerobacter cloacae UW-C83, Escherichia coli K-12, Candida utilis UW-3, and Saccharomyces carlsbergensis UW-110 grown aerobically on several carbon sources are reported. The Y(atp) concept is discussed in relation to the yields on oxygen and to assumed P to O ratios for bacteria and reported P to O ratios for yeast.

High hydrostatic pressure inactivation of Escherichia coli, Pseudomonas fluorescens, Listeria innocua, Staphylococcus aureus, and Lactobacillus helveticus were studied. These microorganisms were inoculated at a concentration between 10(7) and 10(8) cfu/ml in Ringer solution and in ovine milk adjusted to 0, 6, and 50% fat content to evaluate the baroprotective effect of fat content on inactivati...

The plant growth-promoting rhizobacterium Pseudomonas fluorescens WCS374r produces several iron-regulated metabolites, including the fluorescent siderophore pseudobactin (Psb374), salicylic acid (SA), and pseudomonine (Psm), a siderophore that contains a SA moiety. After purification of Psb374 from culture supernatant of WCS374r, its structure was determined following isoelectrofocusing and tan...

In the biocontrol strain Pseudomonas fluorescens CHA0, the Gac/Rsm signal transduction pathway positively controls the synthesis of antifungal secondary metabolites and exoenzymes. In this way, the GacS/GacA two-component system determines the expression of three small regulatory RNAs (RsmX, RsmY, and RsmZ) in a process activated by the strain's own signal molecules, which are not related to N-...

The bacterial microbiota of plants is diverse, with 1000s of operational taxonomic units (OTUs) associated with any individual plant. In this work, we used phenotypic analysis, comparative genomics, and metabolic models to investigate the differences between 19 sequenced Pseudomonas fluorescens strains. These isolates represent a single OTU and were collected from the rhizosphere and endosphere...

Saprophytic Pseudomonas are common root-colonizing bacteria that can improve plant health. Efficient exploitation of these bacteria in agriculture requires knowledge of traits that enhance ecological performance in the rhizosphere. Here, I describe the development and application of a promoter-trapping technology (IVET) that enables the isolation of Pseudomonas fluorescens genes that show eleva...

Pseudomonas fluorescens CHA0 produces several secondary metabolites, e.g., the antibiotics pyoluteorin (Plt) and 2,4-diacetylphloroglucinol (Phl), which are important for the suppression of root diseases caused by soil-borne fungal pathogens. A Tn5 insertion mutant of strain CHA0, CHA625, does not produce Phl, shows enhanced Plt production on malt agar, and has lost part of the ability to suppr...