Several microorganisms can utilize l-rhamnose as a carbon and energy source through the nonphosphorylative metabolic pathway, in which 1-dehydrogenase (RhaDH) catalyzes NAD(P)+-dependent oxidization of to l-rhamnono-1,4-lactone. We herein investigated crystal structures RhaDH from Azotobacter vinelandii ligand-free, NAD+-bound, NADP+-bound, l-rhamnose- NAD+-bound forms at 1.9, 2.1, 2.4, 1.6 Å r...