The web application PCR Suite is an extension of the primer design program Primer3. It allows the design of primer sets encompassing single nucleotide polymorphisms, all exons of a single gene, all open reading frames in a list of cDNAs or the creation of overlapping PCR products.

We report here a new, sensitive and versatile genomic sequencing method, which can be used for in vivo footprinting and studies of DNA adducts. Starting with mammalian genomic DNA, single-stranded products are made by repeated primer extension; these products are subjected to homopolymeric ribonucleotide tailing at the 3' termini with terminal deoxynucleotidyl transferase and then ligated to a ...

Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material. We describe a new technique, polymerase chain displacement reaction (PCDR), which uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal q...

BACKGROUND Bacterial DNA contamination in PCR reagents has been a long standing problem that hampers the adoption of broad-range PCR in clinical and applied microbiology, particularly in detection of low abundance bacteria. Although several DNA decontamination protocols have been reported, they all suffer from compromised PCR efficiency or detection limits. To date, no satisfactory solution has...

An algebra is introduced which can be considered as a rank 2 extension of the Askey-Wilson algebra. Relations in this are motivated by relations between coproducts twisted primitive elements two-fold tensor product quantum $\mathcal{U}_{q}(\mathfrak{sl}(2,\mathbb C))$. It shown that bivariate $q$-Racah polynomials appear overlap coefficients eigenvectors generators Furthermore, corresponding $q...

Feline immunodeficiency virus (FIV) is a lentivirus that causes AIDS in domestic cats, similar to human immunodeficiency virus (HIV)/AIDS in humans. The FIV accessory protein Vif abrogates the inhibition of infection by cat APOBEC3 restriction factors. FIV also encodes a multifunctional OrfA accessory protein that has characteristics similar to HIV Tat, Vpu, Vpr, and Nef. To examine the role of...

recently pcr is being used more frequently as a diagnostic method to detect m. pneumoniae. we used primer pairs reported by van kuppeveld,13 leng,5 lunerberg,7 an bernet' targeting 16s rrna, pi protein, tuf genes. and a short dna equence (mp5) to evaluate the sensitivity among different pcrs. reoptimization experiments showed that tuf pcr had the highest sensitivity amongst these four pcrs...

BACKGROUND DNA barcoding is a popular tool in taxonomic and phylogenetic studies, but for most animal lineages protocols for obtaining the barcoding sequences--mitochondrial cytochrome C oxidase subunit I (cox1 AKA CO1)--are not standardized. Our aim was to explore an optimal strategy for arachnids, focusing on the species-richest lineage, spiders by (1) improving an automated DNA extraction pr...