Background: In mammalian ovaries, loss of over two-thirds of germ cells happens due to cell death. Nonetheless, the exact mechanism of cell death has yet to be determined. The present basic practical study was designed to detect 3 types of programmed cell death, namely apoptosis, autophagy, and necrosis, in murine embryonic gonadal ridges and neonatal ovaries.Methods: Twenty gonadal ridges and ...

Background The aim of this study was to investigate the effect of applying the 1-mT magnetic field (MF) in the ovarian tissue vitrification. MaterialsAndMethods Ovaries of 6-8 week-old Naval Medical Research Institute female mice randomly were divided into 5 groups: V1: Ovaries that immediately after leaving of the mice body, were examined histologically (control group), V2: Ovaries that withou...

The application of decellularized scaffolds for artificial tissue reconstruction has been an approach with great therapeutic potential in regenerative medicine. Recently, biomimetic ovarian was proposed to reestablish endocrine functions. Despite many decellularization methods proposed, there is no established protocol whole ovaries by detergent perfusion that able preserve macro and microstruc...

STUDY QUESTION Does directional freezing improve the structural and functional integrity of ovarian fragments compared with conventional slow freezing and to whole ovary cryopreservation? SUMMARY ANSWER Compared with slow freezing, the use of directional freezing significantly improves all structural and functional parameters of ovarian fragments assessed in vitro and, overall, whole ovaries ...

An evaluation of the toxic effects of three organophosphates; monocrotophos, dimethoate and methyl parathion on female reproduction was made by biochemical estimations of cytoplasmic and membrance bound proteins, lipids, phospholipids and cholesterol in the rat ovaries after treatment with their low residual level doses (LD50 1/8-1/5) to three groups of six rats each for 90 days. All the three ...

In the present study was performed to evaluate the follicular growth and hormonal changes after ovarian artery ligation in rats. Fifty six virgin 90-day–old female rats were used. The rats were chosen from 100 female rats by a vaginal smear, which showed that they were synchronized in estrous phase of the menstrual cycle. Animals were kept in seven cages (n=8 in each) at a temperature of 22 ± 2...

Polycystic ovary syndrome (PCOS), characterized by ovarian androgen excess, is the commonest endocrine disorder in women. Obesity increases androgen synthesis, a phenomenon attributed to the accompanying hyperinsulinemia. Our hypothesis was that adipokines, fat cell-derived hormones, play a direct role in modulating ovarian androgen secretion. Therefore, the aims of this study were to explore t...