Helicobacter pylori infections are accompanied with the release of some antigenic material into the stool. Outer membranes proteins (OMP) are among the major antigens of bacteria, which in comparison with other bacterial constituents have less cross-reactivity. In this study, OMP of H. pylori were isolated from 11 clinical isolates and rabbit antiserum against them was used to detect possible a...

The PDZ domains of the trimeric DegS protease bind unassembled outer-membrane proteins (OMPs) that accumulate in the E. coli periplasm. This cooperative binding reaction triggers a proteolytic cascade that activates a transcriptional stress response. To dissect the mechanism of allosteric activation, we generated hybrid DegS trimers with different numbers of PDZ domains and/or protease-domain m...

Treponema pallidum reacts poorly with the antibodies present in rabbit and human syphilitic sera, a property attributed to the paucity of proteins in its outer membrane. To better understand the basis for the syphilis spirochete's "stealth pathogenicity," we used a dual-label, 3-step amplified assay in which treponemes encapsulated in gel microdroplets were probed with syphilitic sera in parall...

Abstract The presence of protein aggregates in numerous human diseases underscores the significance detecting these to comprehend disease mechanisms and develop novel therapeutic approaches for combating disorders. Despite development various biosensors fluorescent probes that selectively target amyloid fibers or amorphous aggregates, there is still a lack tools capable simultaneously both type...

Outer Membrane Protein (OMP) enriched extracts of Pasteurella multocida A:1 (“A”) and local isolate (“L”) obtained from Nutrient rich free cell (FC), Nutrient deficient free cell (DC) and Biofilm cell (BC) growth were analyzed by SDS-PAGE. In general, the OMP profile in all the three growth conditions of “A” was different from that of “L” suggesting that both could be of different serotype. The...

The basis of differentiation of Pseudomonas aeruginosa into the 17 serotypes of the International Antigenic Typing Scheme is differences in an outer membrane glycolipid, lipopolysaccharide (LPS). This observation, together with the high toxicity and pyrogenicity of LPS, has led to the search for alternative "common" antigens for use as vaccines. The relation between the major outer membrane pro...