Isolation of the C5b-9 complex from inulin-activated whole human serum was effected by molecular sieve column chromatography employing Biogel A-15 M, preparative Pevikon block electrophoresis, and removal of low density beta-lipoproteins by flotation in CsCl. The final product was homogeneous upon cellulose acetate strip electrophoresis and analytical ultracentrifugation. Ouchterlony analyses i...

Crude and purified staphylococcal alpha-toxin were used to demonstrate that the platelet-damaging effect of crude alpha-toxin represents a fifth activity of the alpha-toxin molecule. The homogeneity of the purified toxin employed was demonstrated by ultracentrifugation, Ouchterlony, and immunoelectrophoretic methods. Continuous-flow electrophoretic migration studies demonstrated under a variety...

Antibodies were elicited to FAD by using the hapten N-6-(6-aminohexyl)-FAD conjugated to the immunogenic carrier protein bovine serum albumin. Cross-reactivity was determined by Ouchterlony double-diffusion analysis with N-6-(6-aminohexyl)-FAD coupled to rabbit serum albumin. Anti-FAD IgG was partially purified by (NH4)2SO4 precipitation followed by DEAE-cellulose/CM-cellulose and bovine serum ...

A human neutrophil protease, initially termed neutral peptide-generating protease, has been shown to cleave angiotensin II directly from angiotensinogen and has been identified as leukocyte cathepsin G. When purified neutrophils were disrupted by nitrogen cavitation and fractionated by differential centrifugation, 44 and 24% of the angiotensin II-generating activity was in the lysosomal and und...

A newly developed procedure is described for the isolation and purification on a large scale of luciferases from two different bioluminescent bacteria, Pf and MAV. In only three major steps luciferase can be obtained in greater than 95% purity. An improved method for the isolation of subunits in milligram quantities by column chromatography in 5 M urea is also described; upon recombination of t...

Three components of anthrax toxin (edema factor, protective antigen, and lethal factor) were separated and tested singly at three dose levels and in factorial combination (27 treatments) to determine their efficacy ao immunogens in a resistant host (rat) and in a susceptible host (gulnea pig). The efficacy of each component used as an immunogen was determined by its ability to (i) protect again...

The frequency with which bacterial cholangitis complicates choledocholithiasis or benign postoperative biliary strictures has been the subject of several studies (Elkeles and Mirizzi, 1942; Anderson and Priestley, 1951; Edlund, Mollstedt, and Ouchterlony, 1959; Scott and Khan, 1967). The magnitude of the bacterial population present in this condition had, however, been crudely assessed until in...

An extracellular, nonelastolytic, neutral metalloprotease of Serratia marcescens was purified by sequential ammonium sulfate precipitation, hydroxyapatite adsorption chromatography, flat-bed isoelectric focusing, and Sephadex G-100 gel filtration. The protease preparation had a 280/260 nm absorbance ratio of 1.8, was free of detectable amounts of endotoxin, carbohydrate, phosphorus, and other k...

A human retinol-transporting protein has been isolated from the urine of patients with tubular proteinuria. The protein separated into four fractions in the last two purification steps (sulfoethyl Sephadex chromatography and DEAESephadex chromatography). The yield of purified retinolbinding protein @BP) was 33 to 41% of the amount in concentrates of urinary proteins. A substance with an absorba...

Rock fragmentation is one of the desired results of rock blasting. So, controlling and predicting it, has direct effects on operational costs of mining. There are different ways that could be used to predict the size distribution of fragmented rocks. Mathematical relations have been widely used in these predictions. From among three proposed mathematical relations, one was selected in this stud...