نتایج جستجو برای: optimize conditions
تعداد نتایج: 906004 فیلتر نتایج به سال:
BACKGROUND Assembly of heterologous metabolic pathways is commonly required to generate microbial cell factories for industrial production of both commodity chemicals (including biofuels) and high-value chemicals. Promoter-mediated transcriptional regulation coordinates the expression of the individual components of these heterologous pathways. Expression patterns vary during culture as conditi...
Production of correctly folded and biologically active proteins in Escherichiacoli can be a challenging process. Frequently, proteins are recovered as insoluble inclusion bodies and need to be denatured and refolded into the correct structure. To address this, a refolding screening process based on a 96-well assay format supported by design of experiments (DOE) was developed for identification ...
Biomass yields and sporulation of Beauveria bassiana was concerned on culture conditions, environmental factors and cultivation method. We optimized the best culture conditions for biomass yields of B. bassiana IBC1201 with the novel "two-stage" cultivation method as well as orthogonal matrix method. Firstly, we cultured spore suspension on the basal medium (sucrose 19.00 g, soy peptone 4.06 g,...
This perspective article addressed a rising question if optimizing meal timing can help minimize obesity and diabetes (diabesity) via glucose insulin circadian metabolism. Meal properties including timing, sequence, frequency are of metabolic importance in ruminants humans. The modern man consumes fast foods at wrong times the 24-h period. Suboptimal inadequate physical activity impair normal b...
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