A long QT mutation in the cardiac sodium channel, D1790G (DG), shows enhanced flecainide use-dependent block (UDB). The relative importance of open and inactivated states of the channel in flecainide UDB has been controversial. We used a modifiable, inactivation-deficient mutant channel that contains the F1486C mutation in the IFM motif to investigate the UDB difference between the wild-type (W...

Although the tandem pore potassium channel TASK-3 is thought to open and shut at its selectivity filter in response to changes of extracellular pH, it is currently unknown whether the channel also shows gating at its inner, cytoplasmic mouth through movements of membrane helices M2 and M4. We used two electrode voltage clamp and single channel recording to show that TASK-3 responds to voltage i...

The Manning friction coefficient as a one-dimensional (1D) openchannel flow parameter is determined from a series of two-dimensional (2D) simulations of the flow on steep slopes using a computational hydraulic solver derived from a minimal intervention strategy (MIS2D). Computations are also conducted for the oblique dam-break waves to evaluate the performance of MIS2D. The results are compared...

One of the troublesome aspects of numerical modeling is to determine open boundaries for a model and to make sure that the boundary conditions employed at the open boundaries are compatible with physical processes being simulated within the interior of the model domain. In this investigation the accuracy of the water level data derived from delft3D employing nesting procedure were studied. Thes...

Open channel block (OCB) is a process by which ions bind to the inside of a channel pore and block the flow of ions through that channel. Repulsion of the blocking ions by membrane depolarization is a known mechanism for open channel block removal. For the N-methyl-D-aspartate (NMDA) channel, this mechanism is necessary for channel activation and is involved in neuronal plasticity. Several type...

Analytical solutions have been obtained for both conservative and non-conservative forms of one-dimensional transport and transport-dispersion equations applicable for pollution as a result of a non-conservative pollutant-disposal in an open channel with linear spatially varying transport velocity and nonlinear spatially varying dispersion coefficient on account of a steady unpolluted lateral i...

The fourth transmembrane segment (S4) has been shown to function as a voltage sensor in voltage-gated channels. On membrane depolarization, a stretch of S4 moves outward and initiates a number of conformational changes that ultimately lead to channel opening. Conserved proline residues are in the middle of the S4 of motifs I and III in voltage-dependent Ca2+ channels. Because proline often intr...

Each subunit in a homopentameric Cys-loop receptor contains a specialized coupling region positioned between the agonist binding domain and the ion conductive channel. To determine the contribution of each coupling region to the stability of the open channel, we constructed a receptor subunit (α7-5-HT(3A)) with both a disabled coupling region and a reporter mutation that alters unitary conducta...