It has been demonstrated that Xenopus laevis oocytes are sensitive to catechol­ amines. The oocyte response is characterized as being of beta adrenergic nature and is electrophysiologically measured as a transient increase in potassium con­ ductance (hyperpolarizing current in holding potential —40 mV) (VanRenterghem et al.1985).The aim of this paper was to study the decline of the oocyte respo...

Cold- and deep-water species such as Molva show low resilience to anthropogenic pressures, they become particularly vulnerable at the warm edges of their distribution. In this study, poorly documented Mediterranean ling (Molva macrophthalma) population from northwestern Sea was analysed. This area is considered a cul-de-sac in sea-warming hotspot, where M. macrophthalma shows health status expe...

BACKGROUND Human mature oocytes are very susceptible to cryodamage. Several reports demonstrated that vitrification might preserve oocyte better than slow freezing. However, this is still controversial. Thus, larger clinical, biological and experimental trials to confirm this concept are necessary. The aim of the study was to evaluate and compare fine morphological features in human mature oocy...

Background and aims: Various culture media along with some supplements and enrichment materials have been suggested for in vitro maturation. This study aims to investigate the effect of retinoic acid on nuclear maturation of immature mouse oocytes in vitro. Materials and methods: immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units ...

objective: improvements in cancer treatment have allowed more young women to survive. however, many cancer patients suffer from ovarian failure. cryopreservation is one of the solutions for fertility restoration in these patients. the cryopreservation of isolated follicles is a more attractive approach in the long term. many endocrine and paracrine factors can stimulate the granulosa cells of p...

OBJECTIVE Methoxsalen is a natural photoactive compound which is found in many seed plants. A number of epidermal proliferative disorders can be treated by methoxsalen along with long wave ultraviolet A (UVA). MATERIALS AND METHODS In an experimental study, we aimed to demonstrate the effect of methoxsalen, UVA and their combination on oogenesis Balb/C mice. There were two experimental groups...

Gurken signals from the oocyte to the adjacent follicle cells twice during Drosophila oogenesis; first to induce posterior fate, thereby polarising the anterior-posterior axis of the future embryo and then to induce dorsal fate and polarise the dorsal-ventral axis. Here we show that Gurken induces two different follicle cell fates because the follicle cells at the termini of the egg chamber dif...

Objective:In vitro maturation is a highly effective method for achieving mature  oocytes to treat infertility based on in vitro methods (IVF-ICSI). This technique is effective for treating women who suffer from polycystic ovary syndrome (PCO) and cancer. MATERIALS AND METHODS: immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units of...

the aim of the study was to compare the parthenogenetic activation and in vitro fertilization (ivf) of in vitro matured caprine oocytes. a total of 881 cumulus-oocyte complexes (coc’s) were collected from 243 ovaries. oocytes were matured in tcm-199 medium containing ecg (20 iu/ml), hcg (20 iuμg/ml), oestradiol-17β (1 μg/ml), bsa embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...

introduction: melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. this study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. materials and methods: preantral follicles with diameters of...