A method for fluorescent labeling of PCR products has been developed. This method consists in a two-step procedure in which a first exponential classical PCR is followed by a "linear amplification". This second step relies on incorporation of fluorescent dNTP (dUTP or dCTP) in order to label the product on only one strand. The products can be applied without prior purification directly to a gel...

background and aims: newcastle disease virus (ndv) is an avian paramyxovirus (a-pmv 1) and one of the major pathogens in poultries. vaccination is intended to control the disease, nevertheless this virus is a growing threat to the poultry industry. so, early detection of the virus can prevent the spread of illness and avoid huge economic losses. towards this goal, in this research, we developed...

The lattice Boltzmann method (LBM) is a numerical simplification of the Boltzmann equation of the kinetic theory of gases that describes fluid motions by tracking the evolution of the particle velocity distribution function based on linear streaming with nonlinear collision. If the Bhatnagar–Gross–Krook (BGK) collision model is invoked, the velocity distribution function in this mesoscopic desc...

As society places greater demands on technology, it becomes increasingly important to be able to simulate that technology. While simulation tools can be very sophisticated and capable of handling very complex devices and structures, the cost paid in computation time can be prohibitive. To reduce this time, a number of one-step schemes have been developed, but these schemes suffer from a setup t...