The isolation and characterization of a genomic clone encoding proteinase inhibitor II of potato (Solanum tuberosum) is described. The structure of this gene was determined by sequencing a genomic fragment of about 2 kb containing the entire RNA coding as well as about 900 nucleotides of the 5'-upstream and 250 nucleotides of the 3'-downstream region. The transcription start site was determined...

PARK initially reported z the accumulation of amino acid containing nucleotides in penicillin-treated Staphylococcus aureus. Subsequent work% s has shown that the structure of one of these nucleotides is UDP-GNAc-lactyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala. Nucleotides lacking one or more of these amino acids ("incomplete peptides") have also been isolated from this organism. A similar nucleotide cont...

The 3' end of Sindbis virus minus-sense RNA was tested for its ability to bind proteins in mosquito cell extracts, using labeled riboprobes that represented different parts of this region. We found four domains in the first 250 nucleotides that could bind the same 50- and 52-kDa proteins, three with high affinity and one with low affinity, whereas tested domains outside this region did not bind...

the synthesis of 5' - deoxynucleoside 5' - phosphonates which contains a 5' - cp bond in place of the 5' -cop bond of the naturally occuring nucleotides is described. the preparation of phosphonate derivatives of acyclo - nucleosides and a simple method for the conversion of 1-? -d-ribofuranosyl pyrimidines to the corresponding 1-? -d-arabinofuranosyl pyrimidines are also ex...

A systematic mutagenesis study of the "10-23" DNA enzyme was performed to analyze the sequence requirements of its catalytic domain. Therefore, each of the 15 core nucleotides was substituted separately by the remaining three naturally occurring nucleotides. Changes at the borders of the catalytic domain led to a dramatic loss of enzymatic activity, whereas several nucleotides in between could ...

Diversity of T cell receptor (TCR) genes is primarily generated by nucleotide insertions upon rearrangement from their germ line-encoded V, D and J segments. Nucleotide insertions at V-D and D-J junctions are random, but some small subsets of these insertions are exceptional, in that one to three base pairs inversely repeat the sequence of the germline DNA. These short complementary palindromic...