The synthesis and enzymatic incorporation into RNA of the hydrogen bond degenerate nucleoside analogue 6-(beta-d-ribofuranosyl)-3, 4-dihydro-8H-pyrimido[4,5-c]-[1,2]oxazin-7-one (P) is described. The 5'-triphosphate of this analogue is readily incorporated by T3, T7 and SP6 RNA polymerases into RNA transcripts, being best incorporated in place of UTP, but also in place of CTP. When all the urid...

The interaction of amino pyrrolopyrimidine nucleotides with enzymes catalyzing different steps in the reaction sequence leading to the incorporation of amino acids into polypeptides has been studied. None of the analogue nucleotides could function as the energy source for activation of amino acids or esterification of transfer RNA (tRNA) by mammalian or bacterial enzymes. Toyocamycin S’-triphos...

Introduction: Renewal of Interest in the Treatment of Chronic Hepatitis B Antiviral therapy studies of hepatitis B were first published in the mid 1970s, nearly a decade before the first treatment trials were reported for hepatitis C. Yet much of the emphasis on antiviral therapy in the United States until very recently has been focused on the treatment of hepatitis C. This is due to a number o...

S-Adenosylmethionine (AdoMet) synthetase (EC 2.5.1.6), which catalyzes the synthesis of AdoMet from methionine and ATP, is the major methyl donor for transmethylation reactions and propylamino donor for the biosynthesis of polyamines in biological systems. We have reported previously that wild-type C-1300 murine neuroblastoma (wMNB) cells, made resistant to the nucleoside analogue (Z)-5'-fluoro...

Herpes simplex virus type 1 (HSV-1) causes recurrent herpes labialis (RHL), a common disease afflicting up to 40% of adults worldwide. Mathematical models are used to analyze the effect of antiviral treatment on the transmission of, and the prevalence of drug resistance in, HSV-1 in the United States. Three scenarios are analyzed: no antiviral use, the current level of use, and a substantial in...

Marburg virus (MARV) is a causative agent of severe hemorrhagic fever with high fatality rates endemic in central Africa. Current outbreaks MARV Equatorial Guinea and Tanzania underline the relevance as public health emergency pathogen. In 2021, first known human case was confirmed Guinea, West Since no infectious could be isolated from that fatal we generated recombinant (rec) by reverse genet...

The development of biophysical systems that enable an understanding of the structure and ligand-binding properties of G-quadruplex (GQ)-forming nucleic acid sequences in cells or models that mimic the cellular environment would be highly beneficial in advancing GQ-directed therapeutic strategies. Herein, the establishment of a biophysical platform to investigate the structure and recognition pr...

42280. Source: A Tseng, Pharm D, Immunodeficiency Clinic, The Toronto Hospital, Department of Pharmacy, University of Toronto; S Walmsley, MD, Immunodeficiency Clinic, The Toronto Hospital, Department of Medicine, University of Toronto, Toronto ON. MORE ON MANAGEMENT STRATEGIES FOR CANDIDATES FOR PROTEASE INHIBITORS AND REQUIRING TREATMENT FOR MYCOBACTERIUM TUBERCULOSIS In the preceding article...

A protein with a molecular mass of 42 kDa (P42) from Mycoplasma mobile, one of several mycoplasmas that exhibit gliding motility, was shown to be a novel NTPase (nucleoside triphosphatase). Although the P42 protein lacks a common ATP-binding sequence motif (Walker A), the recombinant proteins expressed in Escherichia coli certainly hydrolysed some nucleoside triphosphates, including ATP. The re...