Methods of preparation and certain properties of the "nucleoproteins" of the following organisms are described: gonococcus, meningococcus, Micrococcus catarrhalis, R pneumococcus, Streptococcus hemolyticus, Staphylococcus aureus. No essential differences between the nucleoproteins and the intact cells of gonococcus and meningococcus were observed in their ability to engender immune substances (...

The human immunodeficiency virus type 1 (HIV-1) Vif protein plays a critical role in the production of infectious virions. Previous studies have demonstrated the presence of small amounts of Vif in virus particles. However, Vif packaging was assumed to be nonspecific, and its functional significance has been questioned. We now report that packaging of Vif is dependent on the packaging of viral ...

Negative staining of virions and isolated nucleoids from avian myeloblastosis virus, murine leukemia virus, murine mammary tumor virus, and feline leukemia virus reveals common internal structures. The majority of virions that are penetrated by phosphotungstate show spherical nucleoids with no apparent symmetry. In a small percentage of virions, two distinctive structures are found: (i) single ...

The DNA unwinding element (DUE) is a sequence rich in adenine and thymine residues present within the origin region of both prokaryotic and eukaryotic replicons. Recently, it has been shown that this is the site where bacterial DnaA proteins, the chromosomal replication initiators, form a specific nucleoprotein filament. DnaA proteins contain a DNA binding domain (DBD) and belong to the family ...

LeuO protein relieves bacterial gene silencer AT8-mediated transcriptional repression as part of a promoter relay mechanism found in the ilvIH-leuO-leuABCD gene cluster. The gene silencing activity has recently been characterized as a nucleoprotein filament initiated at the gene silencer. In this gene locus, the nucleoprotein filament cis-spreads toward the target leuO promoter and results in t...

The influenza viruses contain a segmented, single-stranded RNA genome of negative polarity. Each RNA segment is encapsidated by the nucleoprotein and the polymerase complex into ribonucleoprotein particles (RNPs), which are responsible for virus transcription and replication. Despite their importance, information about the structure of these RNPs is scarce. We have determined the three-dimensio...

SUKENO, N., OTSUKI, Y., KONNo, J. , YAMANE, N., ODAGIRI, T., ARIKAWA, J. and ISHIDA, N. Anti-Nucleoprotein Antibody Response in Influenza A Infection. Tohoku J. exp. Med., 1979 , 128 (3). 241-249 The nucleoprotein (NP) antigen isolated from influenza A virus by solubilization with Triton X-100 (TX-100) and further electrophoresis with SDS-cellulose acetate membrane gave a single band on SDS-pol...

Lohmann, K. (1929). Biochem. Z. 202, 466. Lohmann, K. & Meyerhof, 0. (1934). Biochem. Z. 273, 60. Mejbaum, W. (1939). Hoppe-Seyl. Z. 258, 117. Meyerhof, 0. & Junowicz-Kocholaty, L. (1943). J. biol. Chem. 149, 71. Needham, D. M. & van Heyningen, E. W. (1935). Biochem. J. 29, 2040. Rapoport, S. (1937). Biochem. Z. 291, 429. Roe, J. H. (1934). J. biol. Chem. 107, 15. Sacks, J. (1944). Amer. J. Phy...

Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related sta...