Quercus petraea and Quercus robur are two closely related oak species that frequently hybridize. We sequenced 70 clones containing the 5.8S and ITS2 regions of ribosomal DNA (rDNA) from these two species and did not detect a species-specific difference. Surprisingly, three divergent (up to 12.6%) rDNA families were identified in both species, indicating that they predate the speciation event. D...

In this study, the karyotype, C-heterochromatin blocks, and nucleolar organizer regions (NORs) of four specimens of Lepus europaeus from Turkey were examined. The diploid number of chromosomes (2n), the fundamental number of chromosome arms (FN), and the number of autosomal arms (FNa) were determined as 48, 90, and 86, respectively. All of the chromosomes have been determined to be possessed of...

Oral Squamous cell carcinoma (OSCC) accounts for over 90% of oral cancer cases and a small percentage of these cases are thought to develop from potentially malignant disorders. Main etiological factor associated with OSCC is smoking. It’s a well known fact that in chronic smokers nuclear alterations are evident. There is a correlation between nucleolar function, size and cell doubling time in ...

Active nucleolar organizer regions (NORs) are affine to silver (Ag) and therefore visible in silver stained cell specimens. These so called AgNORs are of diagnostic relevance for early cancer diagnosis. Therefore we seek to automatically detect and segment these dark, spot-like regions. This is challenging, since the contrast between the AgNORs and the nucleus background varies heavily from cel...

Abstract Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). The NOR is constituted multiple, highly repetitive rDNA genes, encoding catalytic ribosomal core RNAs which transcribed from 45S units. Their precise sequence information and organization remain uncharacterized. Here, using a combination long- ...

The aim of this study was to define the relation between silver stained nucleolar organizer regions (AgNORs) count and histological grade, and clinical stage in needle biopsy specimens of 65 prostatic adenocarcinomas. Histological grade was determined according to the modified Gleason system. AgNORs were evaluated by the counting method on routine histological sections stained with silver. Ther...

Banded chromosomes of a male marbled polecat, Vormela peregusna, from the Konya province in Turkey were studied. Conventional chromosome staining, Ag-NOR staining and C-banding were used to reveal the detailed morphology of the karyotype. The karyotype included eight metacentric, four submetacentric , five subtelocentric and one acrocentric autosomal pair (NFa = 70). The X chromosome was a medi...

The ovaries of newly metamorphosed Xenopus females contain oocytes in all stages of early meiotic prophase. In pachytene nuclei extrachromosomal nucleolar DNA appears in the form of a thin cap covering one side of the nucleus. During pachytene this cap of DNA enlarges to occupy half the nucleus. After pachytene the nucleus grows rapidly and the cap of DNA disperses into numerous tiny granules w...

introduction: nucleolar organizer region associated proteins are a group of non histone proteins which act in r-dna transcription and r-rna processing. the amount of these proteins increase in transition from g1 to s phase in cell cycle. hence these proteins can show the proliferative index of the cells. material & methods: for this purpose 40 patients (30 gastritis, 10 carcinoma) with average ...

For a clade that includes Antilope, Gazella,Nanger and Eudorcas (Antilopinae), X;BTA5 translocation is a synapomorphy. Using a combination of fluorescence in situ hybridization (FISH) probes and polymerase chain reaction techniques, we provide (i) the first insight into the X;BTA5 architecture which differs in the species under study: Antilope cervicapra (genus Antilope), Gazella leptoceros (ge...