Background The present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. MaterialsAndMethods Sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. Isolated spermatogonial cells were co-cultured with Sertoli cells in minimal essential medium (...

(1) Background: Stem cells in combination with scaffolds and bioactive molecules have made significant contributions to the regeneration of damaged bone tissues. A co-culture system can be effective enhancing proliferation rate osteogenic differentiation stem cells. Hence, aim this study was investigate human adipose derived when co-cultured osteoblasts seeded on polycaprolactone (PCL):hydroxya...

BACKGROUND Progerin is a mutant form of lamin A responsible for Hutchinson-Gilford progeria syndrome (HGPS), a premature aging disorder characterized by excessive atherosclerosis and vascular calcification that leads to premature death, predominantly of myocardial infarction or stroke. The goal of this study was to investigate mechanisms that cause excessive vascular calcification in HGPS. ME...

background: collagen, the most abundant protein in the human body, and as an extracellular matrix protein, has an important role in the fiber formation. this feature of the collagen renders  establishment of the structural skeleton in tissues. regarding specific features associated with the collagen, such as, formation of the porous structure, permeability and hydrophilicity, it can also be use...

this investigation aimed to examine the protective function of vitamin e on rat bone marrow mesenchymal stem cells (mscs) treated with cadmium chloride. rat bone marrow mscs were extracted using flashing-out and cultured in dmem containing 10% fbs and 100 u/ml pen/strep. at the end of the third passage, cells were divided into 4 groups including control, cadmium chloride, cadmium chloride + vit...

background: clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. however, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. this study was performed to investigate whether...

Tissue nonspecific alkaline phosphatase, promoting arterial calcification in experimental models, is a powerful predictor of total and cardiovascular mortality in general population and in patients with renal or cardiovascular diseases. For this study, to evaluate a possible correlation between serum alkaline phosphatase levels and endothelial function, assessed by strain gauge plethysmography,...

background: periodontal ligament stem cells (pdlscs) are considered as a type of mesenchymal stem cell that is beneficial target for numerous clinical applications in periodontal tissue regeneration therapy. materials and methods: this study examined the effects of dexamethasone (dex) on human pdlscs in vitro. pdlscs obtained from the roots of patient’s teeth were cultured with dex (0.01 μm), a...