Pump-probe microscopy provides molecular information by probing transient, excited state dynamic properties of pigmented samples. Analysis of the transient response is typically conducted using principal component analysis or multi-exponential fitting, however these methods are not always practical or feasible. Here, we show an adaptation of phasor analysis to provide an intuitive, robust, and ...

Relatively little experimental and theoretical data exist on the retinal hazards of ultrashort laser pulses operating in the visible and near infrared spectral regions. Because of potential nonlinear effects that can occur from high-peak irradiance, ultrashort laser pulses propagate from the cornea to the retina, we have developed four projects within our Ultrashort Pulse Effects program. First...

Ultraviolet-visible spectroscopy readily discerns the two types of melanin pigments (eumelanin and pheomelanin), although fundamental details regarding the optical properties and pigment heterogeneity are more difficult to disentangle via analysis of the broad featureless absorption spectrum alone. We employed nonlinear transient absorption spectroscopy to study different melanin pigments at ne...

In this work, numerical estimations of a nonlinear hyperbolic bioheat equation under various boundary conditions for medicinal treatments tumor cells are constructed. The heating source components in transfer model, such as the rate blood perfusions and metabolic generations, considered experimentally temperature-dependent functions. Due to nonlinearity governing relations, finite element metho...

In recent years many works have indicated that a series of interesting nonlinear acoustical effects have been observed in the range of biomedical intensities and frequencies. This new phenomenon in medical ultrasound has attracted immediate interest of many researchers. Two topics on this aspect will be presented in this paper. (1) The acoustical nonlinearity parameter imaging in reflection mod...

H2O as a PET-tracer offers the opportunity to examine perfusion of blood into tissue non-invasively (cf. [1]). It features a short radioactive half-life (≈ 2 min.) and therefore adds a smaller radiation exposure to the patient in comparison to other tracers. The disadvantages arising from the short radioactive half-life are noisy, low-resolution reconstructions. Previous algorithms first recons...

In this paper, we present a ghost cell/level set method for the evolution of interfaces whose normal velocity depend upon the solutions of linear and nonlinear quasi-steady reaction-diffusion equations with curvature-dependent boundary conditions. Our technique includes a ghost cell method that accurately discretizes normal derivative jump boundary conditions without smearing jumps in the tange...

We provide a new algorithm to quantify myocardial blood flow (MBF) from H2O PET data. The new algorithm differs from existing algorithms by taking into account the temporal correlation of the data sets. H2O as a PET-tracer offers the opportunity to examine perfusion of blood into tissue non-invasively. It features a short half-time (≈ 2 min.) and therefore adds a smaller radiation burden to the...