Our previous studies have demonstrated that naturally occurring peptide, Nisin possess antibacterial activity and did not interfere with rabbit vaginal mucosa. In this study, the reproductive toxicity of the Nisin in male rats was evaluated. Rats were fed orally with Nisin (10, 25, and 50 mg/kg/day) for 13 weeks. No treatment related mortality was observed. The body weight gain, food consumptio...

The interaction of the peptide antibiotic nisin with liposomes has been studied. The effect of this interaction was analyzed on the membrane potential (inside negative) and the pH gradient (inside alkaline) in liposomes made from Escherichia coli phosphatidylethanolamine and egg phosphatidylcholine (9:1, wt/wt). The membrane potential and pH gradient were generated by artificial ion gradients o...

Lactic acid alone and its combined application with nisin were evaluated for reducing population of naturally occurring microorganisms on chilled shrimp. Fresh shrimps were dipped in 0, 1.0% and 2.0% (v/v) lactic acid alone and their combined application with 0.04 (g/L/kg) nisin solution for 10 min. Total plate counts of aerobic bacteria (TPCs), Psychrotrophic counts, population of Pseudomonas ...

it is possible to develop new biodegradable active packaging by coating biopolymers on paper-based packaging material and also incorporating proper antimicrobial agents into coating structure. in this study bacterial contamination of two commercial cardboard were evaluated.in order to manufacture an appropriate antibacterial packaging material, coating of chitosan-poly vinyl alcohol blends with...

materials and methods a multivariate variance experiment was performed. to assess the effect of essential oil, nisin and the incubation temperature on growth probability (log p%) of s. typhimurium and s. aureus, four concentrations of c. cyminum l. essential oil (0, 15, 30 and 45 µl/100 ml), three concentrations of nisin (0, 0.5 and 1.5 µg/ml) and three storage temperatures (10, 25 and 35°c) we...

Two Lactococcus lactis subsp. lactis strains, NCK400 and LJH80, isolated from a commercial sauerkraut fermentation were shown to produce nisin. LJH80 was morphologically unstable and gave rise to two stable, nisin-producing (Nip+) derivatives, NCK318-2 and NCK318-3. NCK400 and derivatives of LJH80 exhibited identical morphological and metabolic characteristics, but could be distinguished on the...

Lantibiotics are believed to have a conceivable potential be used as therapeutics, especially against clinically resistant bacterial strains. However, their low solubility and poor stability under physiological conditions limit availability for clinical studies further pharmaceutical commercialization. Nisin is readily available cheap lanthipeptide thus serves good model in the search tools eng...

background and objectives:plant essential oils are sometimes considered for use as antimicrobial agents in foods and medicines and they could be combined with other antimicrobial agents to strengthen the effect and/or reduce the required dose. this study was conducted to determine the chemical composition of the ocimum basilicum, salvia officinalis and trachyspermum ammi essential oils and eval...