The redifferentiation, proliferation, and hyaline-specific extracellular matrix (ECM) protein synthesis of chondrocytes cultured in a polycaprolactone (PCL) scaffold were analyzed. Gene expression of the type II collagen and aggrecan was assessed by real-time PCR in cells from PCL scaffolds, monolayer, and pellet cultures. The proliferative activity was assessed using Ki-67 immunodetection, and...

This study aims to develop a novel device with nanofiber membrane capable of sustained release of temozolomide (TMZ) and neuron growth factor (NGF). An improved bio-availability of TMZ and NGF in surroundings proximal to the device was expected to be attained for a prolonged period of time. The device was developed by integrating TMZ-doped polycaprolactone (PCL) nanofiber (TP) membrane and NGF-...

Hydrogel optimisation for biofabrication considering shape stability/mechanical properties and cell response is challenging. One approach to tackle this issue is to combine different additive manufacturing techniques, e.g., hot-melt extruded thermoplastics together with bioplotted cell loaded hydrogels in a sequential plotting process. This method enables the fabrication of 3D constructs mechan...

Objective(s): Fabrication of scaffolds with improved mechanical properties and favorable cellular compatibility is crucial for many tissue engineering applications. This study was aimed to improve mechanical and biological properties of polycaprolactone (PCL), which is a common biocompatible and biodegradable synthetic polymer in tissue engineering. Nanofibrillated chitosan (NC) was used as a n...

Tracheal stenosis can become a fatal condition, and current treatments include augmentation of the airway with autologous tissue. A tissue-engineered approach would not require a donor source, while providing an implant that meets both surgeons' and patients' needs. A fibrous, polymeric scaffold organized in gradient bilayers of polycaprolactone (PCL) and poly-lactic-co-glycolic acid (PLGA) wit...

The aim of vascular tissue engineering (VTE) is to fabricate tissues that are both mechanically and biologically competent similar to the native vessel they are intended to replace. To this end, the incorporation of sufficient extracellular matrix elastin and collagen is important. The objective of this thesis work was to evaluate the effect of two biochemical factors, L-ascorbic acid (AA) and ...

Bone tissue engineering requires a well-designed scaffold that can be biodegradable, biocompatible, and support the stem cells to osteogenic differentiation. Porous polycaprolactone (PCL) scaffold prepared by fused deposition modeling is an attractive biomaterial that has been used in clinic. However, PCL scaffolds lack biological function and osteoinductivity. In this study, we functionalized ...

In this work, fluorescent gold nanocluster (AuNC) decorated polycaprolactone (PCL) nanofibers (AuNC*PCL-NF) for real time visual monitoring of Hg(2+) detection at ppt level in water is demonstrated. The resultant AuNC*PCL-NF exhibiting remarkable stability more than four months at ambient environment and facilitates increased accessibility to active sites resulting in improved sensing performan...

This paper explores the use of selective laser sintering (SLS) for the generation of bone tissue engineering scaffolds from polycaprolactone (PCL) and PCL/tricalcium phosphate (TCP). Different scaffold designs are generated, and assessed from the point of view of manufacturability, porosity and mechanical performance. Large scaffold specimens are produced, with a preferred design, and are asses...

The next-generation sutures should provide in situ monitoring of wound condition such as temperature while reducing surgical site infection during closure. In this study, functionalized nanodiamond (FND) and reduced graphene oxide (rGO) into biodegradable polycaprolactone (PCL) are incorporated to develop a new multifunctional suture with capabilities. Incorporation FND rGO PCL enhances its ten...