نتایج جستجو برای: nested polymerase chain reaction nested
تعداد نتایج: 707924 فیلتر نتایج به سال:
The analysis of air-passengers’ choices of departure airport in multi-airport regions is a crucial component of transportation planning in many large metropolitan areas, and has been the topic of an increasing number of studies over recent years. In this paper, we advance the state of the art of modelling in this area of research by making use of a Cross-Nested Logit (CNL) structure that allows...
background and purpose: pneumocystis pneumonia, caused by pneumocystis jirovecii, is a fatal disease threatening patients with aids or immunosuppression. assessment of colonization in these patients is of great significance, since it can lead to severe pulmonary disorders. considering the scarcity of published reports on pneumocystis jirovecii isolates from patients in mashhad, iran, we aimed t...
Trematodes are important in economic and public health. Ornithobilharzia turkestanicum (O. turkestanicum) is one of the important economic trematodes in domestic animals. Ornithobilharzia infection in intermediate host (Lymnaea gedrosiana ) can be detected by either exposing snails to light to induce cercarial shedding or by squeezing them between glass slides to detect parasites. The current...
BACKGROUND The graft-versus-leukemia effect is able to induce clinical responses in patients with chronic lymphocytic leukemia treated with a reduced intensity conditioning regimen, followed by allogeneic stem cell transplantation. We investigated whether molecular remissions could be attained after reduced intensity conditioning and allogeneic stem cell transplantation in patients with relapse...
BACKGROUND It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS Analysis of published DNA sequence of mucosal HPV types to select sequences of new...
Polyhydroxybutyrate (PHB) is a polyhydroxy alkanote (PHA) which produces in ralstonia eutropha by three enzymes, including Beta-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase and PHB synthase. These three enzymes are encoded by phbA, phbB and phbC respectively. There is considerable interest in Polyhidroxybutirate, since it can be used as biodegradable plastic, which under optimal cond...
In situ PCR and in situ reverse transcription PCR (RT-PCR) were applied to discriminate between latent and productive infection of human cytomegalovirus (HCMV) in leukocytes. We investigated 28 samples, in which viral pp65 antigen was detected only in the cytoplasm of leukocytes. Additionally we assayed 12 specimens lacking pp65 antigen. Using nested PCR (nPCR), viral DNA was detected in 27 sam...
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