an experiment was carried out to compare the effects of laying hen’s diet supplemented with inorganic and different organic sources of selenium (se) on quality and oxidative stability of eggs during storage. a total of 81, (35-week old) laying hens of lohmann lsl-white were assigned to cages in a completely randomized design with 9 groups of treatment and 3 replicates of 3 birds. hens in each g...

We present a technique to train acoustic models for a target language using speech data from distinct source languages. In this approach, no native training data from the target language is required. The acoustic model candidates for each targetlanguage phoneme are automatically selected from a group of existing source languages by means of a combined phoneticphonological (CPP) metric, develope...

Positive charge at lysine residues of hen egg white lysozyme (HEWL) was neutralized through carbamylation reaction and its effect on the conformational stability of the enzyme was studied. Additionally, guanidination of HEWL was also performed, in which the modified derivative retained the positive charge at lysine residues, but with the addition of guanidino group. About 88% and 85% of lysine ...

We propose a mechanism, based on a > or =10-micros molecular dynamics simulation, for the surprising misfolding of hen egg-white lysozyme caused by a single mutation (W62G). Our simulations of the wild-type and mutant lysozymes in 8 M urea solution at biological temperature (with both pH 2 and 7) reveal that the mutant structure is much less stable than that of the wild type, with the mutant sh...

Hierarchical organization of free energy landscape (FEL) for native globular proteins has been widely accepted by the biophysics community. However, FEL of native proteins is usually projected onto one or a few dimensions. Here we generated collectively 0.2 milli-second molecular dynamics simulation trajectories in explicit solvent for hen egg white lysozyme (HEWL), and carried out detailed con...

Peptidoglycan from Neisseria gonorrhoeae RD5 was completely degraded by hen egg white lysozyme and was not extensively O-acetylated. In contrast, peptidoglycans from gonococcal strains FA19 and FA102 (a penicillin-resistant mutant derived from FA19), were markedly resistant to digestion by hen egg white lysozyme and were extensively O-acetylated.

Bovine α-lactalbumin (αLA) has four disulfide (SS) bonds in the native form (N). On the oxidative folding pathways of this protein, two specific SS folding intermediates, i.e., (61-77, 73-91) and des[6-120], which have two and three native SS bonds, respectively, accumulate predominantly in the presence of Ca2+. In this study, we reinvestigated the pathways using a water-soluble cyclic selenoxi...

The aim of the experiment was to explore the possibility of using free choice feeding technique under diff erent cage density for unselected native chicken. Eighty hens of laying Wareng chicken were divided into two feeding systems. Half of them were fed complete commercial diet of 17% crude protein, with 2800 kcal ME/kg and the other half were subjected to free choice diet. The diets were in m...

We studied the antigen-presenting capacity of mouse L fibroblasts transfected with genes encoding Ia polypeptides of the major histocompatibility complex (MHC). These cells function as efficient antigen-presenting cells (APC) in stimulating peptide antigen-specific MHC-restricted proliferation of long-term T-cell lines, thus establishing the capacity of Ia-expressing L-cell transfectants to pre...

It has been well established that in the oxidative folding of hen egg white lysozyme (HEL), which has four SS linkages in the native state (N), three des intermediates, i.e., des[76-94], des[64-80], and des [6-127], are populated at 20 °C and N is dominantly formed by the oxidation of des[64-80] and des[6-127]. To elucidate the temperature effects, the oxidative folding pathways of HEL were rei...