Head-on collision dynamics of 10, 50 and 100 nm droplets are investigated in vacuum by molecular dynamics, involving 35,858, 4,506,410 36,051,466 molecules, respectively. A variety droplet observed, such as coalescence, hole formation shattering, a function the Weber number. It is found for first time that reflexive separation can occur nanodroplet regime when diameter reaches but not 10 or dro...