The enzyme spermidine/spermine N1-acetyltransferase (N1-SAT) is rapidly induced by heat shock in CHO and A549 cells, with activity declining by 24 h. Depletion of intracellular polyamines by alpha-difluoromethylornithine, an inhibitor of ornithine decarboxylase, blocks this induction. Re-addition of putrescine to these cultures restores the response to heat shock, with a concomitant increase in...

1,2-Dimethylhydrazine (DMH) is a potent procarcinogen with selectivity for the colon. Recently, it has been demonstrated that levels of N1-acetylspermidine were elevated 2-3-fold in colonic tumors induced by this agent compared to control tissues. To determine whether alterations in the urinary levels of this acetylated polyamine or other polyamines were useful biochemical markers for colon can...

BACKGROUND Non-small cell lung cancer (NSCLC) patients with N1 disease have variable outcomes, and additional prognostic factors are needed. The number of positive lymph nodes (LNs) has been proposed as a prognostic indicator. However, the number of positive LNs depends on the number of LNs examined from the resection specimen. The lymph node ratio (LNR) can circumvent this limitation. The purp...

Hel-N1 and HuD belong to the elav gene family and encode neuron-specific RNA-binding proteins that are temporally regulated in neural development. Recently, these genes have been detected in small cell lung carcinoma, a neuroendocrine tumor, with HuD down-regulated in poorly differentiated, variant subsets. We, therefore, sought to determine: (a) the extent to which Hel-N1 and HuD are expressed...

The goal of this study was to examine the neural encoding of voice-onset time distinctions that indicate the phonetic categories /da/ and /ta/ for human listeners. Cortical Auditory Evoked Potentials (CAEP) were measured in conjunction with behavioral perception of a /da/-/ta/ continuum. Sixteen subjects participated in identification and discrimination experiments. A sharp category boundary wa...

Toward the mavacurane and akuammilane monoterpene indole alkaloids, we developed divergent oxidative couplings between nucleus (at N1 or C7) C16-malonate of a common tricyclic model related to strictosidine according biosynthetic hypothesis postulated by Hesse Schmid. These cyclizations led selectively formation N1–C16 bond pleiocarpamine C7–C16 strictamine. We were then able obtain scaffold ta...