The enzymatic activities for the synthesis of phosphatidylcholine from phosphatidylethanolamine and Sadenosyl-L-methionine have been solubilized from rat liver microsomes by ultrasonic disruption in the presence of 0.2% Triton X-100. Significant co-purification of the activities was achieved by chromatography of the Triton extract on octyl-Sepharose CL-4B and, subsequently, on Sepharose 6B. Thr...

From the standpoint of early interventions for dementia, a convenient method of diagnosis using biomarkers is required for Alzheimer's disease (AD) in the early stage as well as amnesic mild cognitive impairment (aMCI). Focusing on differences in DNA methylation due to AD and aMCI, in the present study, we first conducted genome-wide screening, measuring blood DNA methylation levels by the Illu...

PURPOSE To identify novel endometrial cancer-specific methylation markers and to determine their association with clinicopathologic variables and survival outcomes. EXPERIMENTAL DESIGN Differential methylation hybridization analysis (DMH) was done for 20 endometrioid endometrial cancers using normal endometrial DNA as a reference control. Combined bisulfite restriction analysis (COBRA) was us...

CT screening for lung cancer reduces mortality, but will cost Medicare ∼2 billion dollars due in part to high false positive rates. Molecular biomarkers could augment current risk stratification used to select smokers for screening. Gene methylation in sputum reflects lung field cancerization that remains in lung cancer patients post-resection. This population was used in conjunction with cance...

Brother of the Regulator of Imprinted Sites (BORIS/CTCFL) is an autosomal cancer germline (CG) or cancer-testis antigen gene and paralog of CTCF that has been proposed to function as an oncogene in human cancer via dysregulation of the cancer epigenome. Here we show that genetic disruption of DNA methylation in human cancer cells induces BORIS expression, coincident with DNA hypomethylation and...

It is well established that patients with silicosis are at high risk for lung cancer; however, it is difficult to detect lung cancer by chest radiography during follow-up treatment of patients with silicosis because of preexisting diffuse pulmonary shadows. The purpose of this study is to evaluate the usefulness of detection of serum DNA methylation for early detection of lung cancer in silicos...

conclusions the polymorphism and promoter methylation of rassf1a may be a significant factor in hcc, and can be an indicator for poor prognosis in patients with hcc. background the functional and prognostic significance of ras association domain family 1a gene (rassf1a) on hepatocellular carcinoma (hcc) has not been well characterized. objectives this study aimed to investigate the association ...

In this study, we investigate the impact of N-methylation on electronic and photophysical properties both homoleptic heteroleptic Ru(II) bis-terpyridine complexes based recently reported ligand 4’-(4-bromophenyl)-4,4’’’: 4’’,4’’’’-dipyr-idinyl-2,2’ : 6’,2’’-terpyridine (Bipytpy), with pyridine substituents in 4- 4’’-position. The first reduction methylated takes place at pyridinium site is obse...

W e investigated in vivo labelling of D N A in single organs of female Wistar rats after application of 3H-methionine. In 40 g and 120 g rats the tritium incorporation into D N A was 10 times higher in spleen than in liver, kidney and heart. D N A methylation in newborn rats (6 — 8 g) was more pronounced in each of these organs. W e demonstrated a varying degree of tritium in corporation into t...

The O(6)-methylguanine-methyltransferase (MGMT) promoter methylation status is a predictive parameter for the response of malignant gliomas to alkylating agents such as temozolomide. First clinical reports on treating brain metastases with temozolomide describe varying effects. This may be due to the fact that MGMT promoter methylation of brain metastases has not yet been explored in depth. The...