Blastomere fate and embryonic genome activation (EGA) during human embryonic development are unsolved areas of high scientific and clinical interest. Forty-nine blastomeres from 5- to 8-cell human embryos have been investigated following an efficient single-cell cDNA amplification protocol to provide a template for high-density microarray analysis. The previously described markers, characterist...

Fasciclin-like arabinogalactan proteins (FLAs) are involved in numerous important functions in plants but the relevance of their complex structure to physiological function and cellular fate is unresolved. Using a fully functional fluorescent version of Arabidopsis thaliana FLA4 we show that this protein is localized at the plasma membrane as well as in endosomes and soluble in the apoplast. FL...

The availability of L-ascorbic acid labeled with Cl4 had made possible studies on the metabolism of the vitamin in man (l), guinea pig (2,3), and rat (4, 5). In the present investigation the metabolic fate of carboxyllabeled n-ascorbic acid and carboxyl-labeled L-ascorbic acid was compared in guinea pigs and rats. This study is of interest, since little is known of the biological fate of D-asco...

A product analysis was m ade for the reaction between som e aromatic am ines and 3-bromocamphor (2), which was invariably transformed into camphor (3) and eventually aryliminocamphor. The tertiary N ,N -dim ethylanilines were oxidized through a com plex sequence o f couplings and rearrangements to a number o f diam ines of the diphenylm ethane type. Aniline (4) was also successful in the reduct...

Neural stem cells (NSCs) possess high proliferative potential and the capacity for self-renewal with retention of multipotency to differentiate into brain-forming cells. Several signaling pathways have been shown to be involved in the fate determination process of NSCs, but the molecular mechanisms underlying the maintenance of neural cell stemness remain largely unknown. Our previous study sho...

Dopaminergic neurons play important roles in animal behavior, including motivation, reward and locomotion. The Drosophila dopaminergic H-cell interneuron is an attractive system for studying the genetics of neural development because analysis is focused on a single neuronal cell type. Here we provide a mechanistic understanding of how MP3, the precursor to the H-cell, forms and acquires its ide...

During asymmetric cell division, cell fate determinants localize asymmetrically and segregate into one of the two daughter cells. In Drosophila neuroblasts, the asymmetric localization of cell fate determinants to the basal cell cortex requires aPKC. aPKC localizes to the apical cell cortex and phosphorylates the cytoskeletal protein Lethal (2) giant larvae (Lgl). Upon phosphorylation, Lgl diss...

Korach-André, M., Y. Burelle, F. Péronnet, D. Massicotte, C. Lavoie, and C. Hillaire-Marcel. Differential metabolic fate of the carbon skeleton and amino-N of 13Cand [15N]alanine ingested during prolonged exercise. J Appl Physiol 93: 499–504, 2002. First published April 5, 2002; 10.1152/japplphysiol.01195.2001.—The decarboxylation/oxidation and the deamination of 13Cand [15N]alanine ingested (1...

Background: The chick embryo is a classical model to study embryonic development. However, most researchers have not studied the effect of embryonic manipulation on chick hatchability. To determine the effect of egg orientation and type of sealing film on the hatchability of cultured embryos, and to determine the fate of adulte stem cells injected into the blastoderm. Materials and Methods: Win...